远志提取物改善老年大鼠记忆功能及对海马、皮质氧化自由基代谢的影响

Radix Polygalae extracts improve memory function and oxidative free radical metabolism in hippocampus and cortex of aged rats

目的研究远志提取物对于老年大鼠记忆功能的改善作用及对其海马、皮质氧化自由基代谢的影响。方法购买24只24月龄的老年Wistar大鼠,随机均分为2组,分别为老年非用药组、老年用药组,另购买12只6月龄的青年大鼠作为对照组。老年非用药组与对照组均不予任何干预,老年用药组给予远志提取物灌胃,维持3个月,对比各组大鼠记忆功能及海马体、大脑皮质氧化自由基代谢指标:一氧化氮(NO)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)水平。结果各组大鼠第5天逃避潜伏期、穿越平台次数、平台象限停留时间、有效区停留时间、游泳路程差异均有统计学意义(F=187.631、58.387、36.394、198.254、185.436,P<0.05);海马与大脑皮质组织中NO、MDA、SOD、CAT水平差异均具有统计学意义(海马:F=239.196、43.478、625.187、56.438;大脑皮质:F=147.183、36.527、597.514、67.502;P<0.05);进一步对比,对照组青年大鼠各项指标均优于其他各组,其次为老年用药组,差异均有统计学意义(P<0.05)。结论远志提取物能够改善老年大鼠记忆功能,其机制为增强了老年大鼠脑海马、大脑皮质的氧化自由基代谢功能,发挥了更为有效的抗氧化应激损伤、抗衰老功效。

Objective To study how Radix Polygalae extracts improve memory function and their effect on the metabolism of oxidative free radicals in the hippocampus and cortex of aged rats.Methods Twenty-four Wistar rats aged 24 months were purchased and randomly divided into two groups:the blank group and Radix Polygalae group,while another 12 young rats aged 6 months served as the control group.The blank group and control group were not given any intervention,but the Radix Polygalae group was lavaged with Radix Polygalae extracts for 3 months.Memory function,oxide free radical metabolism indexes of the hippocampus and cerebral cortex,nitric oxide(NO),malondialdehyde(MDA),superoxide dismutase(SOD),and catalase(CAT)levels were compared between the three groups.Results There was significant difference in escape latency,number of times the platform was crossed,retention time of platform quadrant,retention time in the effective zone and the distance of swimming between the three groups on the 5 th day(F=187.631,58.387,36.394,198.254,185.436;P<0.05).The levels of NO,MDA,SOD and CAT in the hippocampus and cerebral cortex of rats in each group were significantly different(hippocampus:F=239.196,43.478,625.187,56.438;cerebral cortex:F=147.183,36.527,597.514,67.502;P<0.05).Further comparison showed that each indicator of young rats in the control group was better than that of the other two groups,with statistically significant difference(P<0.05).Conclusions Radix Polygalae extracts can effectively improve the memory function of aged rats,the mechanism of which is to enhance the oxidative free radical metabolism in the hippocampus and cerebral cortex,with better anti-oxidative stress injury and anti-aging effect.