生物信息学分析IGF-2R在乳腺癌中的作用机制

THE ROLE OF IGF-2R IN BREAST CANCER WAS ANALYZED BY BIOINFORMATICS

目的确定IGF-2R基因对乳腺癌的调控作用,挖掘参与乳腺癌发生发展潜在的基因及通路。方法利用“GEOquery”“idmap2.0”“Limma”“Clusterprolifer”等R包筛选乳腺肿瘤组织与正常组织的差异表达基因,并进行GO和KEGG分析。采用STRING、GEPIA和UALACN数据库绘制蛋白互作网络,分析IGF-2R基因与乳腺癌Hub基因的相关性,以及IGF2R表达水平与乳腺癌患者基本情况的相关性。结果筛选得到128个上调差异表达基因,256个下调差异表达基因;IGF-2R基因与核心基因ASPM、RRM2相关性显著;IGF-2R基因表达水平在乳腺癌组织中显著降低,差异有统计学意义(P<0.05);IGF-2R表达水平与患者年龄(20~80岁)、临床分期(Ⅰ、Ⅱ、Ⅲ)、绝经情况和淋巴结转移密切相关,差异有统计学意义(P<0.05)。IGF-2R基因的表达水平仅在Luminal型(LuminalA型、Luminal B-HER2阴性型)乳腺癌中显著降低,差异有统计学意义(P<0.05)。结论IGF-2R通过与ASPM和RRM2正相关参与乳腺癌的调控作用,有望为乳腺癌的防治提供新的思路。

Objective We explored the potential genes and pathways involved in the development of breast cancer to determine the role of IGF-2R gene in breast cancer.Methods The differentially expressed genes between breast cancer and normal tissues were screened by"GEOquery","idmap2.0","Limma","Clusterprolifer"and other R packages,and GO and KEGG analysis were performed.String,gepia and ualacn databases plot protein interaction networks,and analyze the correlation between IGF-2R gene and breast cancer hub gene,and the correlation between IGF2R expression level and basic conditions of breast cancer patients.Results The 128 up-regulated differentially expressed genes and the 256 down-regulated differentially expressed genes were obtained from fruit screening.IGF-2R gene was significantly correlated with core genes ASPM and RRM2;The expression level of IGF-2R gene was significantly decreased in breast cancer(P<0.05).The expression level of IGF-2R was closely related to the patient’s age(20-80 years),clinical stage(I,II,III),menopause and lymph node metastasis,and the difference was statistically significant(P<0.05).The expression level of IGF-2R gene was significantly decreased only in luminal type(luminal A and luminal b-her2 negative)breast cancer,and the difference was statistically significant(P<0.05).Conclusions IGF-2R participates in the regulation of breast cancer through positive correlation with ASPM and RRM2,which is expected to provide new ideas for the prevention and treatment of breast cancer.