鹤草酚介导caspase 3相关凋亡抑制前列腺癌细胞在体内外的增殖

AGRIMOPHOL MEDIATED CASPASE 3 ASSOCIATED APOPTOSIS INHIBITS THE PROLIFERATION OF PROSTATE CANCER CELLS IN VITRO AND IN VIVO

目的探讨鹤草酚对前列腺癌细胞增殖、细胞周期和凋亡的影响。方法通过细胞计数、菌落形成实验、细胞周期实验、细胞凋亡实验等检测细胞增殖、细胞周期和细胞凋亡的变化。通过蛋白印迹法(western blotting)检测细胞凋亡相关蛋白的表达。通过荷瘤裸鼠异种移植瘤模型研究鹤草酚在体内对肿瘤发生的影响。结果鹤草酚抑制了DU145细胞和PC-3细胞的增殖,降低了细胞存活率。与对照组相比,经鹤草酚处理后,S期DU145细胞和PC-3细胞的比例增加,而G2/M期细胞的比例降低。鹤草酚通过激活caspase 3相关的线粒体依赖性凋亡通路,促进DU145细胞和PC-3细胞凋亡。体内实验证实,鹤草酚能减缓前列腺癌细胞的形成,延长移植瘤荷瘤裸鼠的生存时间。结论鹤草酚是一种潜在抗癌药物,它通过激活凋亡通路,来抑制前列腺癌细胞的增殖和细胞周期进展。

Objective To investigate the effects of agrimophol on the proliferation,cell cycle and apoptosis of prostate cancer cells.Methods The changes of cell proliferation,cell cycle and apoptosis were detected by cell count,colony formation test,cell cycle test and apoptosis test.Western blotting was used to detect the expression of apoptosis related proteins.The effect of Crane Herb Phenol on tumorigenesis in vivo was studied by xenograft tumor model in mice.Results Crane grass phenol inhibited the proliferation of DU145 cells and PC-3 cells,and reduced the cell survival rate.Compared with the control group,DU145 cells and PC-3 cells in S phase after treatment with Crane Herb Phenol.The proportion of cells increased,while the proportion of cells in G2/M phase decreased.Crane grass phenol promotes apoptosis of DU145 cells and PC-3 cells by activating caspase 3 related mitochondrial dependent apoptosis pathway.The in vivo experiments have proved that Crane Herb Phenol can slow down the formation of prostate cancer cells and prolong the survival time of transplanted tumor mice.Conclusions Agrimophol is a potential anticancer drug that inhibits the proliferation of prostate cancer cells and the progression of cell cycle by activating the apoptotic pathway.