基于转录组学分析miR-34b/c与miR-449对小鼠输出小管结构与功能的影响

Effects of miR-34b/c and miR-449 on the morphology and function of efferent ductules in mice: A transcriptomics-based analysis

目的:研究miR-34b/c、miR-449在维持输出小管正常结构与功能中发挥的作用,探索miR-34b/c^(-/-)、miR-449^(-/-)双敲除小鼠不育的分子机制。方法:HE染色对小鼠输出小管形态进行观察;利用转录组测序技术对野生型小鼠和miR-34b/c^(-/-)、miR-449^(-/-)双敲除小鼠输出小管基因表达进行分析,从中筛选出miR-34b/c、miR-449可能的靶基因与差异表达基因进行联合分析;qRT-PCR法验证测序结果。结果:双敲除小鼠输出小管形态异常;与野生型小鼠相比,双敲除小鼠输出小管中参与纤毛等结构形成以及水、离子、蛋白质转运等活动的基因表达显著下降。结论:miR-34b/c、miR-449的缺失导致双敲除小鼠输出小管结构异常,输出小管纤毛运动与重吸收功能障碍,导致双敲除小鼠不育。

Objective:To investigate the role of miR-34 b/c and miR-449 in maintaining the normal structure and function of efferent ductules and explore the molecular mechanism of infertility in miR-34 b/c^(-/-)and miR-449^(-/-)dKO mice.Methods:We observed the morphology of mouse efferent ductules by HE staining and analyzed the gene expressions in the efferent ductules of the wild-type and miR-34 b/c^(-/-)and miR-449^(-/-)dKO mice by RNA sequencing.Then we screened the possible target genes of these two miRNA clusters and analyzed them along with the differentially expressed genes,followed by verification of the sequencing results by qRT-PCR.Results:Compared with the wild-type,the dKO mice showed morphologically abnormal efferent ductules and significantly decreased expressions of the genes involved in the formation of cilia and related to the transportation of water,ion and protein in the efferent ductules.Conclusion:The deletion of miR-34 b/c and miR-449 led to morphological abnormality of efferent ductules and dysfunction of aberrant cilia motility and reabsorption in the efferent ductules of dKO mice,resulting in infertility.