Gef26对果蝇原代细胞F-actin的调控作用

Gef26 regulates F-actin organization in Drosophila primary culture

目的:探讨Gef26对果蝇原代细胞发育的影响。方法:培养野生型、gef26突变体(gef266/+和gef263/+)以及gef266/+;Abl4/+双突变体果蝇胚胎的原代细胞,观察细胞形态。对细胞骨架F-actin进行荧光染色,观察细胞骨架的分布。结果:在果蝇胚胎的原代细胞中,野生型肌肉细胞形态正常,gef26突变体的肌肉细胞形态明显异常。gef266/+;Abl4/+双突变体肌肉细胞形态正常,即阿贝尔森酪氨酸激酶(Abelson tyrosine kinase,Abl)突变挽救gef26突变体肌肉细胞形态异常的表型。野生型肌肉细胞中的F-actin呈现规整的形态和连续排布,而两株gef26突变体肌肉细胞中的F-actin排布却呈现出不规整形态和不连续排布。gef266/+;Abl4/+双突变体细胞中的F-actin呈现出规整的形态和连续的排布,即Abl突变挽救gef26突变体F-actin组装紊乱的表型。结论:Gef26可能通过Abl信号通路调控肌动蛋白细胞骨架的组装。

Objective:To investigate the role of Gef26 in Drosophilia primary culture.Methods:Through primary culture of Drosophilia wide type,gef26 mutants and gef266/+;Abl4/+mutants,the cell shape is observed,and by fluorescent staining of F-actin the distribution of cytoskeleton can be observed.Results:In Drosophila primary culture,wild type muscle cells have normal morphology,and the morphology of muscle cells of the gef26 mutants is obviously abnormal.The gef266/+;Abl4/+mutants muscle cells have a normal morphology,namely the phenotype of gef26 mutant muscle cells with abnormal morphology rescued by Abelson tyrosine kinase(Abl)mutation.F-actin in wild type muscle cells showed a regular morphology and continuous arrangement,while that in the gef26 mutants muscle cells showed irregular morphology and discontinuous arrangement.Meanwhile,F-actin in gef266/+;Abl4/+double mutant cells showed a regular morphology and continuous arrangement,that is,the phenotype of gef26 mutant with disordered F-actin arrangement rescued by Abl mutation.Conclusion:Gef26 regulates cell cytoskeleton organization through Abl signaling during muscle cells development.