维生素A对孤独症模型大鼠行为学的影响

Effects of vitamin A on behavior of autistic model rats

目的:探讨维生素A(vitamin A,VA)对丙戊酸钠(sodium valproate acid,VPA)诱导的孤独症模型大鼠行为学的影响。方法:对孕12.5 d Wistar大鼠进行单次腹腔注射,注射物分别为VPA(600 mg/kg)和等量生理盐水,将出生雄性子鼠分为3组:对照组(VPA组,n=11)、维生素A治疗组(VPA+VA组,n=12)以及生理盐水组(SAL组,n=11)。从生后第7天开始对VPA+VA组大鼠以50000 U/2.5 kg剂量的VA(豆油溶解)灌胃,其余两组予以等量的豆油灌胃,持续7 d。3组大鼠在生后第21天和第49天时进行眶静脉采血,比较3组血清VA浓度变化;3组大鼠生后第35天开始按序进行开场实验、三室实验、埋珠实验和水迷宫实验,比较3组的行为表现。结果:与SAL组相比,VPA组重复刻板行为增加、社会交往能力下降、自发活动水平增强、空间学习记忆能力减弱(P<0.05);②经VA治疗后,VPA+VA组与VPA组相比,社交能力增强、自发活动水平减轻、空间学习记忆能力增强(P<0.05),重复刻板行为无明显改善(P>0.05)。结论:维生素A能够改善VPA诱导的孤独症模型大鼠的行为学表现。

Objective:This study aims to explore the effect of vitamin A(VA)on the autistic model rats which were induced by sodium valproate(VPA).Methods:The 12.5 d pregnant Wistar rats were given intraperitoneal injection of VPA(600 mg/kg)or normal saline.Then the male offsprings were randomly divided into three groups:control group(VPA,n=11),vitamin A treatment group(VPA+VA,n=12)and saline group(SAL,n=11).On the 7 th day after birth,rats in the VPA+VA group were given VA(50000 U/2.5 kg)by gavage for 7 days,other two groups were given the same amount of soybean oil by gavage for 7 days.Three groups of rats were tested the vitamin A levels in blood in the the 21 st day and the 49 th day after the birth.The experiments as open-field,three chamber experiment,marble bury and water maze tests in the 35 th day after birth were carried out among 3 groups of rats.Results:Compared with the SAL group,repeated behavior and the spontaneous activity level were increasing significantly in the VPA group,the social intercourse ability,spatial learning and memory ability were decreasing in the VPA group(P<0.05).After vitamin A treatment,compared with the VPA group,the social ability,spontaneous activity,the spatial learning and memory ability were obviously improved in the VPA+VA group(P<0.05).However,there were no significant improvement on the repetitive behavior of VPA+VA group(P>0.05).Conclusion:Vitamin A partially improves the behavior of VPA-induced autistic model rats.