摘要
目的:通过构建基因突变小鼠模型,研究小RNA 2’-O-甲基转移酶Henmt1在小鼠精子发生中的作用。方法:利用CRISPR/Cas9(clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9)技术,构建Henmt1基因突变小鼠,通过计算机辅助精子分析、HE染色及免疫荧光检测等技术对小鼠表型进行分析。结果:成功构建Henmt1基因突变小鼠模型;生育力测试结果提示Henmt1基因突变雄鼠不育;Henmt1基因突变导致雄性小鼠精子发生阻滞;突变小鼠睾丸中逆转座子元件LINE1表达上调。结论:Henmt1对雄性小鼠精子发生有重要调控作用。
Objective:This study aims to observe the role of small RNA 2’-O-methyltransferase Henmt1 during mouse spermatogenesis by generation of Henmt1 mutant mouse model.Methods:Henmt1 mutant mouse were constructed by CRISPR/Cas9 system(clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9)and analyzed the phenotype of mutant mice by computer-aided sperm analysis,HE staining and immunofluorescent staining.Results:Henmt1 mutant mouse model were successfully generated.Henmt1 mutant males are sterility with spermatogenic arrest and up-regulation of retrotransposon LINE1.Conclusion:This study found that HENMT1 plays an important regulatory role during spermatogenesis.
作者
戴馨缘
王仿竹
靳佳川
李伟
沈彬
DAI Xinyuan;WANG Fangzhu;JIN Jiachuan;LI Wei;SHEN Bin(State Key Laboratory of Reproductive Medicine,Nanjing Medical University,Nanjing 211166,China)
出处
《南京医科大学学报(自然科学版)》
CAS
CSCD
北大核心
2020年第9期1263-1268,1296,共7页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金(31622039,31970796)