血浆外泌体源性miRNA在复发性流产患者中的表达初探

The expression of plasma exosomal mi RNA in recurrent spontaneous abortion

目的:通过提取复发性流产(recurrent spontaneous abortion,RSA)患者与正常妊娠女性血浆外泌体中miRNA,运用miRNA测序技术筛选出差异表达的miRNA,以探讨其在复发性流产患者中的表达情况。方法:复发性流产患者及年龄、停经天数相匹配的早期妊娠行人工流产女性各3例,收集其清宫术后的肘前静脉血,提取血浆,对血浆外泌体中的miRNA进行提取、测序,分析miRNA表达谱,并对表达差异较大的miRNA进行靶基因预测及功能分析和信号通路分析。结果:复发性流产患者与正常妊娠女性相比,血浆外泌体中有24个miRNA存在差异表达,其中表达下调的miRNA有11个,表达上调的miRNA有13个。生物功能分析表明这些差异表达miRNA的靶基因可能参与某些妊娠过程从而发挥其功能。结论:复发性流产患者的血浆外泌体中表达的miRNA种类及数量与正常妊娠相比存在差异,此可作为RSA预防诊治中的分子标记物之一,得进一步研究。

Objective:To analyze the differential expression of exosomal miRNAs deriving from plasma between recurrent spontaneous abortion(RSA)and healthy-control subjects. Methods:This study enrolled 3 patients with RSA who were admitted to Nanjing Maternity and Child Health Care Hospital,and a cohort of 3 age and gestation-matched healthy-control subjects. Peripheral blood samples were collected on the first day after curettage surgery. Trancmission electron microscopy was used to identify exosomes derived from plasma samples. Sequencing was performed to identify the differentially expressed exosomal miRNAs between the RSA group and the control group. Target gene prediction,gene ontology analysis,and pathway analysis were performed for miRNAs with significant differential expression. All subjects provided written informed consent for their participation in the study. Results:By comparing with the control group,24 significantly differential expressed exosomal miRNAs were screened in RSA group,of which 11 were down-regulated and 13 up-regulated. Conclusion:The exosomes contain abundant miRNAs which expressions are significantly different. These exosomal differential miRNAs may be a promising biomarker in the activation of RSA,needing further investigation.