ASF1B在肾透明细胞癌中的表达及临床意义

Expression and clinical significance of ASF1B in renal clear cell carcinoma

目的探讨ASF1B基因在肾透明细胞癌患者中的表达水平及对临床预后的意义。方法通过在肿瘤基因图谱(the cancer genome atlas,TCGA)及UCSC Xena数据库下载并分析肾透明细胞癌基因表达数据共607例,其中正常肾组织及癌旁组织数据72例,肾透明细胞癌数据535例。明确ASF1B基因在肾透明细胞癌组织与正常组织间的表达差异,分析ASF1B表达与患者生存时间的相关性。使用生信线上工具(GEPIA、cBioPortal、STRING以及TIMER)探究肾透明细胞癌患者ASF1B表达与基因突变及甲基化水平相关性,研讨其与肿瘤免疫微环境的相互作用,探讨ASF1B对肾透明细胞癌的预测能力。结果ASF1B基因在肾透明细胞癌细胞中表达水平明显高于正常肾组织中的表达水平(P=2.22e^(-16));ASF1B基因高表达组的患者生存周期明显低于ASF1B基因低表达组(P=0.024)。ASF1B基因在经过多因素回归分析(P<0.001)后可确定为肾透明细胞癌的高风险独立预后因子。ASF1B基因突变水平无法作为患者预后的诊断指标;cg06969527甲基化位点的甲基化程度与基因表达呈负相关(P=0.0048,HR=-0.16)。ASF1B基因表达水平与CD4^(+)T细胞表达水平呈负相关(P=5.89e^(-1),Rho=-0.025),与CD8^(+)T细胞表达水平呈正相关(P=2.04e^(-10),Rho=0.29),与CD2基因表达水平呈正相关(P=2.47e^(-21),Rho=0.395),与CD3D基因表达水平呈正相关(P=9.54e^(-22),Rho=0.399),与CD3E基因表达水平呈正相关(P=1.58e^(-20),Rho=0.387)。结论ASF1B基因与肾透明细胞癌患者的不良预后有关,可以作为肾透明细胞癌的独立危险因素。

Objective To investigate the expression level of ASF1B gene in patients with renal clear cell carcinoma and its significance for clinical prognosis.Methods By downloading and analyzing gene expression data from the tumor gene atlas(TCGA)and UCSC Xena database,607 cases of renal clear cell carcinoma were analyzed,including 72 cases of normal renal tissue and paracancerous tissue,and 535 cases of renal clear cell carcinoma.The difference in the expression of ASFIB gene was identified between renal clear cell carcinoma and normal tissues,and the correlation between ASF1B expression and survival time of patients was analyzed.Using online tools such as GEPIA,cBioPortal,STRING,and TIMER,the correlation between ASF1B expression,gene mutation,and methylation levels in patients with renal clear cell carcinoma was explored,and its interaction with the tumor immune microenvironment,and explored the predictive ability of ASF1B in renal clear cell carcinoma was discussed.Results The expression level of ASF1B gene in renal clear cell carcinoma cells was significantly higher than that in normal renal tissues(P=2.22e^(-16)).The survival cycle of patients with high ASF1B gene expression was significantly lower than those with low ASF1B gene expression(P=0.024).ASF1B gene can be identified as a high-risk independent prognostic factor for renal clear cell carcinoma after multivariate regression analysis(P<0.001).The level of ASF1B gene mutation cannot be used as a diagnostic indicator for the prognosis of patients.The degree of methylation at the cg06969527 methylation site was negatively correlated with gene expression(P=0.0048,HR=-0.16).The expression level of ASF1B gene was negatively correlated with the expression level of CD4^(+)T cells(P=5.89e^(-1),Rho=-0.025),positively correlated with the expression level of CD8^(+)T cells(P=2.04e^(-10),Rho=0.29),positively correlated with the expression level of CD2 gene(P=2.47e^(-21),Eho=0.395),positively correlated with the expression level of CD3D gene(P=9.54e^(-22),Rho=0.399),and positively correlated with the expression level of CD3E gene(P=1.58e^(-20),Rho=0.387).Conclusion The ASF1B gene is associated with poor prognosis in patients witii renal clear cell carcinoma,and can be an independent risk factor for renal clear cell carcinoma.