摘要
[Objectives]To explore the effect of total flavonoids from Phyllanthus emblica L.on tumor proliferation and immune function.[Methods]The effects of total flavonoids of P.emblica L.on the proliferation of 6 different tumor cell lines(human hepatoma cell line HepG-2,human cervical cancer cell line Hela,human gastric cancer cell line SGC7901,human nasopharyngeal carcinoma cell line CNE-2,human lung cancer cell line H460 and human ovarian cancer cell line A2780)were compared by SRB method.The effect of total flavonoids from P.emblica L.on the proliferation of mouse lymphocytes induced by concanavalin(ConA)or lipopolysaccharide(LPS)in vitro was detected by CCK-8 method.[Results]The results of SRB assay showed that compared with the normal group,the total flavonoids of P.emblica L.had obvious inhibitory effect on 6 kinds of tumor cells.Among them,the inhibitory effect on H460 cells and CNE-2 cells was the most significant,and the IC50 was(471.36±50.66),(463.26±40.75)μg/mL,respectively.The high dose group of total flavonoids from P.emblica L.had the same inhibitory effect as the positive drug 5-Fu.The results of CCK-8 assay showed that compared with the blank group,the total flavonoids of P.emblica L.significantly inhibited the proliferation of mouse lymphocytes induced by ConA or LPS(*P<0.05,**P<0.01).[Conclusions]The total flavonoids of P.emblica L.had significant anti-tumor activity.
[Objectives] To explore the effect of total flavonoids from Phyllanthus emblica L. on tumor proliferation and immune function. [Methods] The effects of total flavonoids of P. emblica L. on the proliferation of 6 different tumor cell lines（human hepatoma cell line HepG-2, human cervical cancer cell line Hela, human gastric cancer cell line SGC7901, human nasopharyngeal carcinoma cell line CNE-2, human lung cancer cell line H460 and human ovarian cancer cell line A2780） were compared by SRB method. The effect of total flavonoids from P. emblica L. on the proliferation of mouse lymphocytes induced by concanavalin（ConA） or lipopolysaccharide（LPS） in vitro was detected by CCK-8 method. [Results] The results of SRB assay showed that compared with the normal group, the total flavonoids of P. emblica L. had obvious inhibitory effect on 6 kinds of tumor cells. Among them, the inhibitory effect on H460 cells and CNE-2 cells was the most significant, and the IC50 was（471.36±50.66）,（463.26±40.75） μg/mL, respectively. The high dose group of total flavonoids from P. emblica L. had the same inhibitory effect as the positive drug 5-Fu. The results of CCK-8 assay showed that compared with the blank group, the total flavonoids of P. emblica L. significantly inhibited the proliferation of mouse lymphocytes induced by ConA or LPS(*P<0.05,**P<0.01). [Conclusions] The total flavonoids of P. emblica L. had significant anti-tumor activity.
基金
Supported by School-level Project of Guangxi University of Chinese Medicine(XP018091)
Basic Ability Improvement Project of Young and Middle-aged Teachers in Colleges and Universities in Guangxi(2017KY0323).
