摘要
[Objectives]To establish a HPLC method for the simultaneous determination of 14 characteristic components in Gegen Qinlian tablets and the content of Gegen Qinlian tablets in 11 manufacturers.[Methods]A Inertsil ODS-3(250 mm×4.6 mm,5μm)reversed-phase high performance liquid chromatography column was used with acetonitrile-0.02 mol/L ammonium acetate+0.03%triethylamine solution(adjusted with glacial acetic acid to pH of 4.3)as the mobile phase for gradient elution at a flow rate of 1.0 mL/min.The determination wavelength of 8 components(puerarin,daidzin,liquiritin,baicalin,wogonoside,daidzein,ammonium glycyrrhizinate,baicalein)was 250 nm;the determination wavelength of wogonoside was 280 nm;the determination wavelength of 5 components(epierberine,jatrorrhizine hydrochloride,coptisine,palmatine hydrochloride and berberine hydrochloride)was 346 nm.[Results]There was a good linear relationship between the injection volume of puerarin,daidzin,liquiritin,baicalin,wogonoside,epierberine,jatrorrhizine hydrochloride,coptisine,daidzein,palmatine hydrochloride,ammonium glycyrrhizinate,berberine hydrochloride,baicalein and wogonin and peak area(r>0.9990)in the range of 146.26-5850.24,24.13-965.04,18.45-738.00,79.18-3167.32,9.57-382.80,4.76-190.40,2.57-102.80,13.41-536.40,10.60-424.00,11.33-453.22,12.08-483.20,46.73-1869.25,20.28-811.20,12.11-484.50 ng,respectively;the average recovery rates(n=6)were 2.18%,1.79%,1.81%,1.68%,2.27%,2.13%,1.96%,1.07%,0.93%,0.61%,2.92%,0.77%,2.79%and 0.62%,respectively;the precision,repeatability and stability were good,and the RSD was less than 3%.This method was used to determine 16 batches of Gegen Qinlian tablets produced by 11 enterprises.Wogonoside was not detected in a lot of samples,but 14 components were detected for other enterprises,but the content was different.[Conclusions]The method was accurate and feasible and could be used for the overall quality control of this variety.
[Objectives] To establish a HPLC method for the simultaneous determination of 14 characteristic components in Gegen Qinlian tablets and the content of Gegen Qinlian tablets in 11 manufacturers. [Methods] A Inertsil ODS-3(250 mm×4.6 mm, 5 μm) reversed-phase high performance liquid chromatography column was used with acetonitrile-0.02 mol/L ammonium acetate+0.03% triethylamine solution(adjusted with glacial acetic acid to pH of 4.3) as the mobile phase for gradient elution at a flow rate of 1.0 mL/min. The determination wavelength of 8 components(puerarin, daidzin, liquiritin, baicalin, wogonoside, daidzein, ammonium glycyrrhizinate, baicalein) was 250 nm; the determination wavelength of wogonoside was 280 nm; the determination wavelength of 5 components(epierberine, jatrorrhizine hydrochloride, coptisine, palmatine hydrochloride and berberine hydrochloride) was 346 nm. [Results] There was a good linear relationship between the injection volume of puerarin, daidzin, liquiritin, baicalin, wogonoside, epierberine, jatrorrhizine hydrochloride, coptisine, daidzein, palmatine hydrochloride, ammonium glycyrrhizinate, berberine hydrochloride, baicalein and wogonin and peak area(r>0.999 0) in the range of 146.26-585 0.24, 24.13-965.04, 18.45-738.00, 79.18-316 7.32, 9.57-382.80, 4.76-190.40, 2.57-102.80, 13.41-536.40, 10.60-424.00, 11.33-453.22, 12.08-483.20, 46.73-186 9.25, 20.28-811.20, 12.11-484.50 ng, respectively; the average recovery rates(n=6) were 2.18%, 1.79%, 1.81%, 1.68%, 2.27%, 2.13%, 1.96%, 1.07%, 0.93%, 0.61%, 2.92%, 0.77%, 2.79% and 0.62%, respectively; the precision, repeatability and stability were good, and the RSD was less than 3%. This method was used to determine 16 batches of Gegen Qinlian tablets produced by 11 enterprises. Wogonoside was not detected in a lot of samples, but 14 components were detected for other enterprises, but the content was different. [Conclusions] The method was accurate and feasible and could be used for the overall quality control of this variety.
