白藜芦醇通过动力相关蛋白1减轻糖尿病小鼠心肌氧化应激的研究

Effects of resveratrol in reducing oxidative stress of rats with diabetic cardiomyopathy by dynamin-related protein 1

目的观察白藜芦醇(RES)对糖尿病小鼠心肌氧化应激的影响并探讨其分子机制。方法将C57 BL/6小鼠分为对照组、糖尿病组、糖尿病加RES组及糖尿病加RES和腺甘-磷酸(AMP)依赖的蛋白激酶抑制剂组。分别用Western blotting检测各组实验动物心肌动力相关蛋白1(Drp1)、磷酸化的Drp1(pDrp1)、AMP依赖的蛋白激酶(AMPK)、磷酸化的AMPK(p AMPK)蛋白表达;比色法检测超氧化物歧化酶(SOD)活性及活性氧(ROS)含量。结果与对照组比较,糖尿病小鼠心肌组织pDrp1(ser637)、pAMPK表达显著降低,SOD活性减低,ROS含量升高。给予RES处理后可以逆转上述改变,但RES的作用被Compound C所阻断。结论RES可能通过AMPK-Drp1信号通路减轻糖尿病心肌氧化应激。

Objective To investigate into the effects of resveratrol on oxidative stress of rats with diabetic cardiomyopathy and its potential underlying mechanisms.Methods Experimental animals were divided into four groups:control mice,diabetic mice,diabetic mice treated with resveratrol,and diabetic mice treated with resveratrol and AMPK inhibitor Compound C.Expression of Drp1,p Drp1,AMPK and pAMPK was detected by Western blotting,and SOD activity and ROS were detected by the colorimetric method.Results The relative expression of p Drp1(ser637),pAMPK and the activity of SOD were significantly decreased in diabetic cardiomyopathy as compared with the control group.ROS content was significantly increased in diabetic cardiomyopathy as compared with their counterparts.Resveratrol treatment increased the expression of pAMPK,p Drp1(ser637),and the activity of SOD and decreased ROS content.However,the function of resveratrol was blocked by Compound C.Conclusion Resveratrol may reduce oxidative stress of diabetic cardiomyopathy through AMPK-Drp1 signaling pathway.