加味六安煎对咳嗽变异性哮喘豚鼠血清IL-2、IL-12及IL-13水平影响

Effects of Modified Liuan Decoction(加味六安煎)on IL-2,IL-12 and IL-13 Levels in Serum of Guinea Pigs with Cough Variant Asthma

目的观察咳嗽变异性哮喘(cough variant asthma,CVA)模型豚鼠血清白细胞介素-2(interleukin-2,IL-2)、白细胞介素-12(interleukin-12,IL-12)、白细胞介素-13(interleukin-13,IL-13)水平的变化,探讨加味六安煎治疗咳嗽变异性哮喘的作用机制,为临床治疗提供依据。方法将30只雄性豚鼠随机分为正常组,CVA模型组,孟鲁司特钠组,加味六安煎低、中、高剂量组。以卵蛋白、氢氧化铝致敏并吸入卵蛋白激发法复制CVA豚鼠模型,观察各组大鼠咳嗽次数及血清IL-2、IL-12及IL-13水平变化。结果(1)咳嗽次数:与正常组相比,CVA模型组咳嗽次数明显增加(P<0.05);与CVA模型组相比,各治疗组咳嗽次数明显减少(P<0.05);各治疗组组间比较差异无统计学意义(P>0.05)。(2)IL-2:与正常组相比,CVA模型组IL-2水平升高(P<0.05);与CVA模型组相比,各治疗组IL-2水平差异无统计学意义(P>0.05);各治疗组组间比较差异无统计学意义(P>0.05)。(3)IL-12:与正常组相比,CVA模型组IL-12水平升高(P<0.05);与CVA模型组相比,加味六安煎高剂量组IL-12水平降低(P<0.05),孟鲁司特钠组及加味六安煎低、中剂量组差异无统计学意义(P>0.05),各治疗组组间比较差异无统计学意义(P>0.05)。(4)IL-13:与正常组相比,CVA模型组IL-13水平升高(P<0.05);与CVA模型组相比,孟鲁司特钠组差异无统计学意义(P>0.05),加味六安煎各剂量组IL-13水平降低(P<0.05);与孟鲁司特钠组相比,加味六安煎各剂量组IL-13水平降低(P<0.05);加味六安煎各剂量组组间比较差异无统计学意义(P>0.05)。结论加味六安煎可以通过调节IL-2、IL-13及IL-12的水平,调节Th1/Th2的平衡状态,减轻气道炎症水平及气道高反应性,延缓气道上皮下纤维化,进而减少咳嗽次数,改善气道重塑,从而达到防治本病的目的。

Objective To observe the changes of interleukin-2(IL-2),interleukin-12(IL-12)and interleukin-13(IL-13)levels in serum of guinea pig with cough variant asthma(CVA),and to explore the mechanism of Modified Liuan Decoction(加味六安煎)in treating CVA.Methods Thirty male guinea pigs were randomly divided into the control group,the CVA group,the montelukast sodium group,Modified Liuan Decoction low-dose,medium-dose and high-dose groups.The guinea pig were hypersensitized with albumin and aluminium hydroxide to replicate CVA guinea pig models.Cough frequency and serum IL-2,IL-12 and IL-13 levels of guinea pigs in each group were observed.Results(1)Cough frequency:Compared with normal group,CVA group increased significantly(P<0.05),compared with CVA group,the treatment group significantly decreased(P<0.05),there was no statistical difference among the treatment groups(P>0.05);(2)IL-2:Compared with normal group,CVA group significantly inscreased(P<0.05),compared with CVA group,there was no statistical difference among the treatment groups(P>0.05),there was no statistical difference among the treatment groups(P>0.05);(3)IL-12:Compared with normal group,IL-12 levels of CVA group increased(P<0.05),compared with CVA group,IL-12 levels of Modified Liuan Decoction high dose group decreased(P<0.05),there was no statistical difference among the treatment groups(P>0.05);(4)IL-13:Compared with normal group,IL-13 levels of CVA group increased(P<0.05),compared with CVA group,the montelukast sodium group had no significant difference(P>0.05),IL-13 levels of each dose group of Modified Liuan Decoction decreased(P<0.05),IL-13 of each dose group of Modified Liuan Decoction decreased(P<0.05),there was no significant difference among different doses of Modified Liuan Decoction(P>0.05).Conclusion The Modified Liuan Decoction regulate the balance of Th1/Th2 by regulating the levels of IL-2,IL-13 and IL-12.Consequently,it can reduce the airway inflammation and airway hyperreactivity,and then reduce airway subepithelial fibrosis and the number of coughs and improve airway remodeling,so as to prevent and treat this disease.