地麦活性糖改善Ⅱ型糖尿病大鼠药效学研究

Study on the Pharmacodynamics of Dimai Active Carbohydrate in Improving Type Ⅱ Diabetes

目的探讨地麦活性糖对Ⅱ型糖尿病大鼠的药效学研究。方法雄性SD大鼠,高脂饲料喂养4周后,链脲佐菌素造模,血糖合格稳定后,随机分为正常组、高脂模型组、生地组、麦冬组、生地-麦冬(1∶1)组、生地-麦冬(2∶1)组、生地-麦冬(1∶2)组、阳性对照药盐酸二甲双胍组。相应药物干预4周后,考察大鼠体质量、空腹血糖,ELISA法测定血清中白细胞介素-1(IL-1)、白细胞介素-6(IL-6)、肿瘤坏死因子α(TNF-α)水平;全自动生化分析仪检测总胆固醇(TC)、甘油三酯(TG)水平;H&E染色法观察大鼠远端回肠组织形态学改变;免疫组化法观察大鼠回肠L细胞胰高血糖素样肽-1(GLP-1)的阳性表达。结果与高脂模型组相比,地麦各比例组可显著降低空腹血糖,麦冬-生地(1∶1)组血清中IL-1、IL-6降低效果较其他组好,麦冬-生地(1∶1)组和麦冬-生地(2∶1)组均显著降低模型组大鼠血清TG(P<0.05),同时,提高回肠组织的完整性,提高回肠L细胞的GLP-1阳性表达。结论地麦活性糖可降低Ⅱ型糖尿病大鼠血糖,可能通过降低炎性细胞因子、保护回肠组织、提高GLP-1阳性表达来实现。

Objective To study the pharmacodynamics of Dimai active carbohydrate on type 2 diabetic rats.Methods Male SD rats were fed with high-fat diet for four weeks,type 2 diabetic model was established by STZ,and blood glucose was qualified and stable,and they were randomly divided into normal group,high-fat model group,Shengdi group,Maidong group,Shengdi-Maidong(1∶1),ShengdiMaidong(2∶1),Shengdi-Maidong(1∶2),metformin hydrochloride group.After the corresponding drug intervention for 4 weeks,the rats’body weight,fasting blood glucose,and serum levels of IL-1,IL-6,and TNF-αwere determined by ELISA;total cholesterol(TC)and triglycerides(TG)were detected by an automatic biochemical analyzer;H&E staining method to observe the morphological changes of rat distal ileum;immunohistochemical method to observe the positive expression of GLP-1 in rat ileum L cells.Results Compared with the high-fat model group,the different proportions of ground wheat can significantly reduce fasting blood glucose.The reduction effect of IL-1,IL-6 and IL-6 in the serum of MaidongShengdi(1∶1)group is better than other groups.Both the Shengdi(1∶1)group and the Maidong(2∶1)group significantly reduced serum TG in the model group(P<0.05).At the same time,it can improve the integrity of the ileum tissue,and increase the ileum L GLP-1 positive expression of cells.Conclusion The improvement of Dimai active carbohydrate is mainly achieved by reducing inflammatory cytokines,protecting ileum tissue,and increasing the positive expression of GLP-1.