血府逐瘀汤对大鼠肾小球内皮细胞增殖和凋亡作用研究

Effect of Xuefu Zhuyu Decoction on the Propagation and Apoptosis in Rat Glomerular Endothelial Cells

目的观察血府逐瘀汤和糖基化终末产物(advanced glycation end products,AGEs)对大鼠肾小球微血管内皮细胞(glomerula endothelial cells,GECs)增殖和凋亡的影响,探讨其相关作用机制。方法对大鼠GECs进行分离培养与鉴定;制备糖基化白蛋白(AGE-BSA)和血府逐瘀汤含药血清;通过MTT法及AV/PI法检测不同浓度AGE对大鼠肾小球内皮细胞增殖、凋亡的影响,同时研究应用血府逐瘀汤含药血清对AGE-BSA上述作用的影响。结果与正常对照组比较,AGEs组RGECs的增殖率降低,细胞凋亡率升高,其作用呈浓度依赖趋势;AGEs可以抑制大鼠肾小球内皮细胞管腔形成;血府逐瘀汤组RGECs的凋亡率较AGEs组下降,可促进内皮细胞的管腔形成。结论 AGEs促进大鼠肾小球内皮细胞的凋亡,抑制内皮细胞的增殖和管腔形成,血府逐瘀汤可抑制AGEs的相关作用。

Objective:To study the effects of Xuefu Zhuyu Decoction and Advanced glycation end products(AGEs)on the apoptosis and propagation in rats glomerular endothelial cells(GECs)and the related mechanism.Methods:Primary RGECs of Sprague-Dawley rats were isolated and cultured.Preparation of AGEs and Xuefu Zhuyu Decoction were made.We took the methods of AV/PI and MTT to study the effects of Xuefu Zhuyu Decoction on the propagation and apoptosis of rats GECs.Results:Compared with the normal control group,the propagation rate of RGECs in AGEs group decreased,the apoptosis rate increased,which depended on its dose denstiny.The Lumen formation of rats GECs was inhibited by AGEs.and the apoptosis rate of RGECs in Xuefu Zhuyu Decoction group was lower than that in AGEs group.Xuefu Zhuyu Decoction can Promote the lumen formation of rats GECs.Conclusion:AGEs promotes the apoptosis of rats GECs and inhibits the propagation and Lumen formation of rats GECs.Xuefu Zhuyu Decoction can inhibit the related effects of AGEs.