肝纤维化大鼠阴虚证评价量表的制定和评价

Study on Evaluation Scale of Yin Deficiency of CCl4 Induced Liver Fibrosis Rat

目的:阴虚证是肝纤维化常见的一种证候,本研究制定肝纤维化大鼠阴虚证的评价量表,为动物肝纤维化模型阴虚证候的评价提供方法。方法:通过CCl4造模法制备肝纤维化大鼠模型,对大鼠的体质量、饮水、甩尾时间等行为学指标进行检测,并对大鼠表征如皮肤毛发、耳轮颜色等进行观察,结合临床阴虚证诊断标准等制定动物阴虚证评价量表,并对量表的信度和效度进行检测。结果:模型组大鼠出现体型消瘦、体温升高、皮肤毛发稀疏无光泽、烦躁易怒、舌红、脉数等症状和体征,差异均具有统计学意义(P<0.05)。量表有很好的内部一致性信度、折半信度、内容效度和结构效度。结论:通过研究,得到肝纤维化大鼠阴虚证的评价量表,且量表的信度和效度都达到标准,可用于大鼠阴虚证模型的评价。

Objective:Yin deficiency syndrome is a common syndrome of hepatic fibrosis.This study provides a method for the evaluation of yin deficiency syndrome in animal liver fibrosis models.Methods:Rat model of hepatic fibrosis was prepared by CCl4 model.Behavioral parameters such as body weight,drinking water,and tail flick time were measured.Rat characterizations such as skin hair and ear color were also observed.Combining with the diagnostic criteria of clinical yin deficiency syndrome,the yin deficiency syndrome evaluation scale was developed and the reliability and validity of the scale were tested.Results:The rats in the model group showed signs of body weight loss,elevated body temperature,sparse and dull skin hair,irritability,red tongue,and pulse number,and the differences were statistically significant(P<0.05).The scale has good internal consistency reliability,split-half reliability,content validity,and construct validity.Conclusion:Through the research,the assessment scale of Yin deficiency syndrome was obtained,the reliability and validity reached the standard of the cale,so it can be used forthe evaluation of the yin deficiency syndrome model in rats.