基于心率变异性探讨“意守”的自主神经系统调控效应

Exploring Regulation Effect of Mind-Keeping on Autonomic Nervous System Based on Heart Rate Variability

目的探讨慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)稳定期患者舌苔微生态多样性的特点。方法对30例COPD稳定期患者和10例健康人舌苔标本进行16S rRNA高通量测序,并对结果进行alpha多样性分析、beta多样性分析和线性判别分析效应量(LEfSe)分析以寻找COPD稳定期患者舌苔微生态特点。结果在OTU水平上,PLS-DA分析显示COPD组和健康对照组的舌苔菌群组成具有显著性差异;颗粒二氧化碳噬纤维菌(capnocytophaga granulosa)可能成为COPD稳定期患者的舌苔标志菌,二氧化碳噬纤维菌属(Capnocytophaga)与患者CAT评分的分值呈正相关关系。弯曲杆菌属(Campylobacter)的丰度与COPD气流受限程度相关。结论COPD患者舌苔微生态失调与COPD疾病的发生发展有一定的相关性,二氧化碳噬纤维菌属和弯曲杆菌属可能是COPD的标志菌群。

Objective To investigate the characteristics of microecological diversity of tongue coating in patients with stable chronic obstructive pulmonary disease(COPD).Methods The high throughput 16 S rRNA sequencing was performed in 30 patients with stable COPD and 10 healthy subjects.Alpha diversity,beta diversity and lefse analysis were performed to find out the microecological characteristics of tongue coating in stable COPD patients.Results PLS-DA analysis showed that there were significant differences in the composition of tongue coating flora between COPD group and healthy control group.Capnocytophaga might be the marker bacteria of tongue coating in patients with stable COPD.Capnocytophaga was positively correlated with CAT score.The abundance of Campylobacter was related to the airflow limitation of COPD.Conclusion The imbalance of tongue coating microecology in patients with COPD is related to the occurrence and development of COPD.Capnocytophaga and Campylobacter may be the marker microorganisms of COPD.