冠心病先天伏寒证血清总胆红素、补体因子的临床研究

Clinical Study on Serum Total Bilirubin and Complement Factor of Different TCM Syndromes of Coronary Heart Disease

目的通过对冠心病伏寒证与其他证型(气滞血瘀、气虚血瘀、心肾阴虚、心肾阳虚)血清中的血脂、总胆红素、补体C3、C4、补体H检测结果的统计分析,找出冠心病伏寒证与不同证候人群血清之间的相关性。方法收集符合诊断及纳入标准的各组人群血清样本共191例,采用双抗体夹心酶联免疫吸附试验(ELISA)法分别检测总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、总胆红素(TB)、补体C3、补体C4、补体H含量。采用SPSS 21.0软件对结果进行统计分析。结果伏寒组与其他各组比较血清TB、补体C3具有显著性差异。补体H在伏寒组与气虚血瘀组、气滞血瘀组之间比较具有显著性差异;补体C4在伏寒组与心肾阳虚组、心肾阴虚组支架比较具有显著性差异;各组间血脂各项指标比较无明显差异,可能与各组人群服用降脂药物有关。结论先天伏寒患者血清中补体C3水平升高、胆红素含量相对较低,可能是这类人群易患冠心病的重要原因。

Objective Through the statistical analysis of the detection results of blood lipid,total bilirubin,complement C3,C4 and H in the serum of coronary heart disease latent cold syndrome and other syndrome types(Qi stagnation and blood stasis,Qi deficiency and blood stasis,heart and kidney Yin deficiency and heart and kidney Yang deficiency),the correlation between coronary heart disease latent cold syndrome and the serum of different syndrome groups was found out.Methods A total of 191 serum samples from each group meeting the diagnostic and inclusion criteria were collected.The contents of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),total bilirubin(TB),complement C3,C4 and H were detected by double antibody sandwich enzyme-linked immunosorbent assay(ELISA).The results were statistically analyzed by SPSS 21.0 software.Results There were significant differences in total bilirubin(TB)and complement C3 between latent cold syndrome group and other syndrome groups.There were significant differences in complement H between latent cold syndrome group,Qi deficiency and blood stasis group,and Qi stagnation and blood stasis group.The scaffold of complement C4 in the latent cold syndrome group was significantly different from that in the Yang deficiency of heart and kidney group and Yin deficiency of heart and kidney group.There was no significant difference in various blood lipid indexes among the groups,which may be related to the use of lipid-lowering drugs in each group.Conclusion The elevated serum complement C3 level and relatively low bilirubin content in congenital hypochondriac patients may be the important reasons for their susceptibility to coronary heart disease.