以中医外治法为基础探讨芦荟联合喉风散对钙剂外渗损伤组织炎症因子的影响

Effect of Luhui(Aloe)Combined with Houfeng Powder(喉风散)on Tissue Inflammation Factors in Calcium Extravasation Injury Based on TCM External Treatment

目的研究以中医外治法为基础探讨芦荟联合喉风散对钙剂外渗损伤组织炎症因子的影响。方法40只健康SPF级SD大鼠进行编号处理后,按照随机数字表法,将所有大鼠分为A组(空白组)、B组(模型组)、C组(硫酸镁组)、D组(芦荟组)、E组(芦荟喉风散组),分组干预。观察动物全身表现,测量损伤面积和组织炎症因子TNF-α浓度。结果各组创面在的TNF-α的浓度在损伤后表现为先上升后下降。在干预后的1 d、4 d、7 d、11 d,A组、B组、C组、D组、E组的TNF-α浓度比较,数据差异有统计学意义(P<0.05)。结论芦荟联合喉风散在损伤早期可以促进TNF-α的迅速释放,损伤后期可一定程度上抑制TNF-α的过度释放。

Objective To establish a rat model of extravasative tissue injury induced by calcium.To explore the effect of Luhui(Aloe)combined with Houfeng Powder(喉风散)on inflammatory factors in tissues damaged by calcium extravasation through animal experiments.Methods Forty healthy SPF SD rats were randomly divided into group A(blank group),group B(model group),group C(magnesium sulfate group),group D(Luhui(Aloe)group)and group E[Luhui(Aloe)combined with Houfeng Powder group]according to the random number table method.The whole body performance was observed,and the injury area and the concentration of tissue inflammatory factor TNF-αwere measured.Results The concentration of TNF-αin wound surface of each group was increased first and then decreased after injury.On the 1 st,4 th,7 th and 11 th day after intervention,the TNF-αconcentrations of group A,B,C,D and E were significantly different(P<0.05).Conclusion Luhui(Aloe)combined with Houfeng Powder can promote the rapid release of TNF-αin the early stage of injury,and inhibit the excessive release of TNF-αto some extent in the late stage of injury.