益气通络排浊颗粒对慢性非细菌性前列腺炎大鼠TXB_(2)、6-K-PGF_(1α)的影响

Effects of Yiqi Tongluo Paizhuo Granules(益气通络排浊颗粒)on TXB_(2) and 6-K-PGF_(1α) in Rats with Chronic Non-Bacterial Prostatitis

目的研究益气通络排浊颗粒对慢性非细菌性前列腺炎(CNP)大鼠血栓素B2(TXB_(2))、6-酮-前列腺素F1α(6-K-PGF_(1α))的影响。方法将56只大鼠随机分为空白组、模型组、中药对照组(前列解毒胶囊)、西药对照组(西乐葆)、益气通络排浊颗粒高剂量组、中剂量组、低剂量组,在大鼠前列腺背侧叶分别注射角叉菜胶溶液0.1 mL,空白组相同位置注射0.9%氯化钠溶液0.1 mL;各组大鼠于术后21 d开始给药,空白组、模型组予生理盐水,连续给药3周后,检测血浆TXB_(2)、6-K-PGF_(1α)及两者比值T/K,病理学观察前列腺组织。结果模型组大鼠前列腺可见上皮细胞形态破坏,间质水肿,明显血管充血及炎性细胞浸润,各治疗组大鼠腺体间质充血、水肿、炎细胞浸润等均有不同程度减轻。与模型组相比,益气通络排浊颗粒高、中、低剂量组大鼠血清TXB_(2)水平明显下降;中剂量组6-K-PGF_(1α)水平上升,高剂量组、中剂量组T/K值下降,差异具有统计学意义(P<0.05);低剂量组6-K-PGF_(1α)及T/K差异无显著性,高剂量组6-K-PGF_(1α)差异无统计学意义(P>0.05),西药对照组血清TXB_(2)水平及T/K值明显下降,6-K-PGF_(1α)水平上升差异具有统计学意义(P<0.05),中药对照组血清TXB_(2)水平明显下降,差异具有统计学意义(P<0.05)。结论益气通络排浊颗粒可降低CNP大鼠血浆TXB_(2)、升高6-K-PGF_(1α),使其比值T/K明显降低,中剂量效果最佳,在提升6-K-PGF_(1α)水平方面优于各对照组。

Objective To study the effects of Yiqi Tongluo Paizhuo Granules(益气通络排浊颗粒)on thrombin B2(TXB_(2))and 6-ketone-prostaglandin F1(6-k-PGF_(1α))in chronic non-bacterial prostatitis(CNP)rats.Methods Fifty-six rats were randomly divided into blank group,model group,traditional Chinese medicine control group[Qianjiexian Jiedu Capsule(前列解毒胶囊)],the western medicine control group(celebrex),Yiqi Tongluo Paizhuo Granules high,middle and low dose groups.The dorsal lobe of the prostate in rats were injected carageen glue solution 0.1 mL.The blank group was given the same position injection of 0.9%sodium chloride solution 0.1 mL.The rats in each group were administrated 21 days after the operation.The blank group and the model group were given normal saline.After 3 weeks of continuous administration,plasma levels of TXB_(2)and 6-k-PGF_(1α)and their ratio T/K were detected,and the prostate tissues were pathologically observed.Results Morphological destruction of epithelial cells,interstitial edema,vascular congestion and inflammatory cell infiltration were observed in the model group,which were alleviated to different degrees in each treatment group.Compared with those of the model group,TXB_(2)levels of the rats in the high,middle and low dose groups were significantly decreased.The level of 6-k-PGF_(1α)was increased in the middle dose group,while the ration of T/K was decreased in the high and middle dose groups,and the difference was statistically significant(P<0.05).There was no significant difference between the low dose group and the high dose group(6-K-PGF_(1α)and T/K).The serum TXB_(2)level and T/K in the western medicine control group were significantly decreased,the serum TXB_(2)level in the western medicine control group was significantly increased(P<0.05),and the serum TXB_(2)level in the traditional Chinese medicine control group was significantly decreased(P<0.05).The difference was statistically significant(P<0.05).Conclusion Yiqi Tongluo Paizhuo Granules can reduce TXB_(2)and increase 6-K-PGF_(1α)in plasma of CNP rats,and the ratio of T/K can be significantly reduced,and the Yiqi Tongluo Paizhuo Granules middle dose group’s effect is the best,which is better than the control group in improving the level of 6-K-PGF_(1α).