SPC25在肝细胞癌中的表达和意义——基于数据库分析

Expression of SPC25 in hepatocellular carcinoma and its clinical significance: a study by bioinformatics analysis

目的:检测纺锤体极体组件25同族体(SPC25)在肝细胞癌(HCC)中的表达及预后价值。方法:下载癌症基因组图谱(TCGA)中肝细胞癌组织和正常肝组织样本的基因表达谱数据和临床数据,比较SPC25在肝细胞癌组织和正常肝组织中的表达,并检测SPC25表达水平与患者生存预后的关系。结果:肝细胞癌组织(n=371)的SPC25表达高于正常肝组织(n=50),P<0.000 1。与配对的肝细胞癌组织(n=50)相比,肝细胞癌组织(n=50)的SPC25表达水平明显升高,P<0.000 1。将肝细胞癌患者(n=365)分为SPC25高表达组和低表达组,高表达组与低表达组相比具有更高比例较差的组织病理学分级(G3/G4:69/138,50%vs 61/222,27.5%,P<0.001)、晚期肿瘤(StageⅢ/Ⅳ:44/131,33.6%vs 43/210,20.5%,P=0.003)和死亡(67/140,47.9%vs 63/225,28%,P=<0.001)。Kaplan-Meier法显示,肝细胞癌患者中SPC25高表达组总体生存率显著低于低表达组,P<0.05。亚组分析证实SPC25表达与病理分级和临床分期的相关性。单因素、多因素回归分析显示,SPC25高表达和晚期肿瘤是影响肝细胞癌患者总体生存率的独立预后因素,P<0.05。结论:SPC25在肝细胞癌组织中较正常肝组织高表达,SPC25可能是肝细胞癌患者预后的独立影响因素。

Objective: To investigate the association of the expression of spindle pole body component 25 homolog(SPC25) with clinical features and prognosis in patients with hepatocellular carcinoma(HCC). Methods: In this study, we attempted to assess the independent prognostic value of SPC25 in terms of overall survival(OS) through a retrospective study based on data from the cancer genome atlas(TCGA) liver and hepatocellular carcinoma(LIHC) cohort. The gene expression profile and related clinical data of HCC tissue samples were collected, and these samples were divided into low SPC25 expression group and high SPC25 expression group. The expression of SPC25 and related clinical information were analyzed. Results: The HCC tissues(n=371) had significantly elevated SPC25 expression than normal liver tissues(n=50). SPC25 expression was higher in HCC tissues(n=50) than in paired adjacent normal tissues(n=50). By grouping patients with primary HCC(n=365) into high and low SPC25 expression groups, we found that the high expression group had a substantially higher proportion of high-grade tumors(G3/G4: 69/138, 50% vs 61/222, 27.5%, P<0.000 1), advanced tumors(stage Ⅲ/Ⅳ: 44/131, 33.6% vs 43/210, 20.5%, P=0.003) and death(67/140, 47.9% vs 63/225, 28%, P=0.000 1) compared with the low expression group. Kaplan-Meier curves of OS indicated that high SPC25 expression was associated with worse survival outcomes. Subgroup analysis confirmed the associations in G1/G2, G3/G4 tumors and in early and advanced stages. Following univariate and multivariate analysis revealed that SPC25 expression and clinical stage was an independent prognostic indicator for poor OS(P<0.05). Conclusion: Based on these findings, we infer that SPC25 was overexpression in HCC tissues and its expression might independently predict poor prognosis.