视觉和嗅觉在桃红颈天牛成虫近距离搜寻和识别配偶过程中的作用

Role of vision and olfaction in short-distance mate location and recognition in Aromia bungii(Coleoptera:Cerambycidae)

【目的】明确视觉和嗅觉在桃红颈天牛Aromia bungii成虫近距离搜寻和识别配偶过程中的作用。【方法】采用半野外行为观测笼的方法,分别设置不同的处理,观察视觉和嗅觉对桃红颈天牛成虫搜索搜寻配偶行为的影响。【结果】桃红颈天牛雌、雄成虫均不能成功定位被蜡封处理的异性;致盲后的成虫识别异性的时间较对照显著增加,但均能和正常异性成功交配;剪除触角的雄虫无法成功定位雌虫,剪除触角的雌虫可与正常雄虫成功交配但交配次数显著降低,近距离识别异性的时间显著增加;雌雄成虫剪除下颚须后对异性搜索时间以及交配次数影响不明显;剪除下唇须后显著影响成虫对异性的搜寻时间以及交配次数。【结论】桃红颈天牛成虫近距离搜寻和识别配偶过程中,雄虫依靠触角摆动主动搜索雌虫;雌雄成虫相遇后主要靠体表信息化学物质互相识别,主要功能器官为触角和下唇须。在此过程中视觉发挥一定作用,但嗅觉起主导作用。

[Objectives]To clarify the role of vision and olfaction in short-distance mate location and recognition in Aromia bungii.[Methods]The influence of vision and olfaction on the mate-searching behavior of A.bungii was observed in captive A.bungii.[Results]Neither adult females nor males could successfully locate wax-sealed mates.Blind adults could successfully mate with normal mates,but the time spent on mate recognition was significantly longer than that required by normal beetles.Males without antennae could not successfully locate females.Females without antennae could successfully mate with normal males,but the number of copulatioins decreased significantly,whereas the time used to recognize mates over a short-distance increased significantly.Removal of the maxillary palpus had no significant effect on mate-searching time or number of copulations in both sexes.However,removal of the labial palpus significantly affected mate-searching time and mating time.[Conclusion]The wiggling of antennae by male A.bungii plays a key role in short-range searching for females.Male and female adults recognize each other mainly by contacting and sensing body surface chemicals,primarily with the antennae and labial palpus.Overall,olfaction plays the dominant role,and vision the secondary role,in short-range mate location and recognition in A.bungii.