摘要
【目的】为研究不同温度条件下草地螟LoxostegesticticalisL.成虫飞行能力的差异,飞行后能源物质含量以及生殖能力的变化。【方法】本实验通过飞行磨系统测试3日龄草地螟成虫在不同温度下10 h内的飞行能力,飞行后通过甘油三酯试剂盒、糖元试剂盒以及蛋白质试剂盒来分别测试其体内甘油三酯、糖元及蛋白的含量,再将飞行后成虫配对测试其生殖能力的变化。【结果】18-22℃条件下草地螟成虫的飞行能力最强,各个温度条件下飞行后甘油三酯及糖元的含量都较对照降低,18和22℃下降幅度最大,然而能源物质的利用效率较高,是其最适宜飞行的温度。各个温度条件下飞行后可溶性蛋白质的含量没有显著差异(P>0.05);不同温度吊飞后生殖能力没有显著差异(P>0.05)。【结论】18℃和22℃是草地螟较适宜的飞行温度。甘油三酯及糖元是草地螟飞行的能源物质。草地螟10h内的飞行不需要付出生殖的代价。
[Objectives]To study the flight capacity,energy content and fecundity of the beet webworm(Loxostege sticticalis L.),at different temperatures.[Methods]Three-day-old adults were tethered for 10h in a flight mill to test their flight capacity at different temperatures,after which their triglyceride,glycogen and protein content were measured using the corresponding assay kit,and their fecundity assessed.[Results]18-22℃was the optimal flight temperature.Triglyceride and glycogen content were significantly lower in flight-tested moths than in the control group and those flight-tested at 18-22℃had the lowest triglyceride and glycogen content.There was no significant difference in protein content and fecundity between flight-tested moths and the control group.[Conclusion]18℃to 22℃was the optimal flight temperature range for the beet webworm,and triglyceride and glycogen are the main energy source for flight.10h of tethered flight did not affect the fecundity of 3-day-old moths.
作者
唐继洪
罗礼智
江幸福
程云霞
张蕾
TANG Ji-Hong;LUO Li-Zhi;JIANG Xing-Fu;CHENG Yun-Xia;ZHANG Lei(State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2023年第6期1669-1678,共10页
Chinese Journal of Applied Entomology
基金
国家重点研发计划项目(2022YFD1400600)
国家绿肥产业技术体系(CARS-22)
农业农村部政府购买服务项目(15226006)
中国农业科学院植物保护研究所基本科研业务费(S2022XM05)
关键词
草地螟
甘油三酯
飞行磨系统
糖元
利用效率
Loxostege sticticalis
Triglyceride
flight mill system
glycogen
utilization efficiency