不同温度吊飞对草地螟飞行能力、能源物质含量及生殖能力的影响

Effect of flight temperature on flight capacity,energy substances and fecundity of the beet webworm(Loxostege sticticalis L.)

【目的】为研究不同温度条件下草地螟LoxostegesticticalisL.成虫飞行能力的差异,飞行后能源物质含量以及生殖能力的变化。【方法】本实验通过飞行磨系统测试3日龄草地螟成虫在不同温度下10 h内的飞行能力,飞行后通过甘油三酯试剂盒、糖元试剂盒以及蛋白质试剂盒来分别测试其体内甘油三酯、糖元及蛋白的含量,再将飞行后成虫配对测试其生殖能力的变化。【结果】18-22℃条件下草地螟成虫的飞行能力最强,各个温度条件下飞行后甘油三酯及糖元的含量都较对照降低,18和22℃下降幅度最大,然而能源物质的利用效率较高,是其最适宜飞行的温度。各个温度条件下飞行后可溶性蛋白质的含量没有显著差异(P>0.05);不同温度吊飞后生殖能力没有显著差异(P>0.05)。【结论】18℃和22℃是草地螟较适宜的飞行温度。甘油三酯及糖元是草地螟飞行的能源物质。草地螟10h内的飞行不需要付出生殖的代价。

[Objectives]To study the flight capacity,energy content and fecundity of the beet webworm(Loxostege sticticalis L.),at different temperatures.[Methods]Three-day-old adults were tethered for 10h in a flight mill to test their flight capacity at different temperatures,after which their triglyceride,glycogen and protein content were measured using the corresponding assay kit,and their fecundity assessed.[Results]18-22℃was the optimal flight temperature.Triglyceride and glycogen content were significantly lower in flight-tested moths than in the control group and those flight-tested at 18-22℃had the lowest triglyceride and glycogen content.There was no significant difference in protein content and fecundity between flight-tested moths and the control group.[Conclusion]18℃to 22℃was the optimal flight temperature range for the beet webworm,and triglyceride and glycogen are the main energy source for flight.10h of tethered flight did not affect the fecundity of 3-day-old moths.