TSWV提高植物游离氨基酸含量促进西花蓟马生殖力的研究

TSWV infection increases the free amino acid content of host plants and promotes the reproduction of Frankliniella occidentalis

【目的】为明确番茄斑萎病毒(Tomatospottedwiltorthotospovirus,TSWV)对西花蓟马Frankliniella occidentalis孤雌生殖力的影响,并探明TSWV通过改变寄主植物营养调控西花蓟马生长发育及生殖力的途径。【方法】通过比较西花蓟马取食健康和感染TSWV曼陀罗叶片后孤雌生殖力的差异,采用生化方法测定了曼陀罗感染TSWV后营养物质含量的变化及添加氨基酸对西花蓟马生长发育及生殖力的影响。【结果】与对照相比,感染TSWV的西花蓟马孤雌生殖力显著提高1.5倍;曼陀罗感染TSWV后其总游离氨基酸含量和可溶性蛋白含量分别是健康植株的1.3倍和3.0倍;与取食未添加氨基酸的对照组相比,取食添加氨基酸叶碟的西花蓟马若虫期发育加快了0.3d;有性生殖后代数量显著增加50%(P<0.01),孤雌生殖后代数量显著增加60%(P<0.01)。【结论】TSWV侵染引起的曼陀罗中总游离氨基酸含量的升高是导致TSWV促进西花蓟马生长发育和繁殖力的重要因素之一。

[Objectives]To determine the influence of tomato spotted wilt orthotospovirus(TSWV)on the parthenogenetic reproduction of Frankliniella occidentalis,and investigate how TSWV modulates host plant nutrition to regulate the development and reproductive capacity of these thrips.[Methods]The parthenogenetic fecundity of F.occidentalis that fed on either TSWV-infected,or uninfected,Datura stramonium leaves,were compared.The nutrient content of infected D.stramonium was determined using biochemical methods and the effects of adding amino acids to the growth and fecundity of F.occidentalis were determined.[Results]The parthenogenetic fecundity of F.occidentalis that fed on infected leaves was significantly(1.5 times)higher than that of the control group.The total free amino acid and soluble protein content of infected D.stramonium plants were 1.3 and 3.0 times higher,respectively,than those of uninfected plants.The nymphal development of F.occidentalis fed on leaf disks supplemented with amino acids was significantly accelerated by 0.3 d,and offspring numbers produced by sexual and parthenogenetic reproduction were 50%(P<0.01)and 60%(P<0.01)higher,respectively,than those of the control group.[Conclusion]TSWV infection increases the total free amino acid content of D.stramonium,which appears to benefit the development and reproduction of F.occidentalis.