厌氧氨氧化微生物的快速富集及微生物群落分析

Quick enrichment of anammox bacteria and its microbial community analysis

为加速厌氧氨氧化微生物(Anammox)的富集过程,在厌氧、35℃和pH值7.2~7.5的条件下,采用2个SBR反应器富集培养Anammox菌,并对微生物群落进行分析。其中,1#反应器第97d获得359.44mgTN/(L·d)的脱氮速率;第121d,浮霉菌门(Planctomycetes)及厌氧氨氧化优势菌属Candidatus Kuenenia分别占比32.10%和30.20%。2#反应器(投加1/1000功能菌源)第42d获得455.36mgTN/(L·d)的脱氮速率;第60d,浮霉菌门(Planctomycetes)和厌氧氨氧化优势菌属Candidatus Kuenenia分别占比45.22%和37.25%,1/1000菌源策略显著加速了Anammox菌的富集过程。

In order to accelerate the process of enrichment of anaerobic ammonia oxidation(Anammox)bacteria,the Anammox sludge was cultured with 2SBR reactors under the conditions of anaerobic,35℃and pH=7.2~7.5,and the microbial community was analyzed.Among them,the 1#reactor achieved a NRR of 359.44 mgTN/(L·d)on day 97;on day 121,the Planctomycetes and Candidatus Kuenenia accounted for 32.10%and 30.20%,respectively.The 2#reactor(added 1/1000functional bacterial source)achieved a NRR of 455.36mgTN/(L·d)on the 42nd day;on the 60th day,the proportion of the Planctomycetesand Candidatus Kueneniaincreased to 45.22%and 37.25%,the 1/1000functional bacterial source strategy accelerated the Anammox enrichment process.