桑黄菌丝体和子实体中次级代谢产物及其活性的比较

Comparison of active secondary metabolites between mycelia and fruiting bodies of Sanghuangporous sanghuang

研究桑黄发酵菌丝体次级代谢产物及活性与子实体的差异性,探讨其替代子实体的可能性。研究通过高效液相色谱分析和化学法比较菌丝体和子实体石油醚、氯仿、乙酸乙酯和正丁醇4个萃取相中的成分差异,以二苯基三硝基苯肼自由基(DPPH)清除率和Trolox当量抗氧化能力(TEAC)作为抗氧化活性的指标、HepG2和MCF-7癌细胞的抑制率作为抑制肿瘤细胞生长的指标,比较其活性差异。结果表明,菌丝体和子实体4个萃取相在化学成分上存在差异;在活性方面,菌丝体各萃取相的抗氧化活性高于子实体,而子实体抗肿瘤活性优于菌丝体。菌丝体醇提取的总黄酮含量高于子实体醇提物,抗氧化活性和总黄酮含量有显著相关性,发酵菌丝体在抗氧化活性方面具有替代子实体的可行性。

The difference of secondary metabolites and their activities between mycelia and fruiting body of Sanghuangporous sanghuang was studied and the possibility of replacing fruiting bodies by mecelia in process of production of the metabolites was evaluated.The difference of metabolite component between mycelium and fruiting bodies fractionated with petroleum ether,chloroform,ethyl acetate and n-butanol and analyzed by high performance liquid chromatography and chemical method was compared.Antioxidant activity of the components extracted including scavenging 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical activity and Trolox equivalent antioxidant capacity(TEAC),and antineoplastic activity including the ability of inhibiting the growth of HepG2 and MCF-7 cancer cells were also evaluated.The result was that the components of the four extractive phases of mycelium and fruiting body were obviously different and the antioxidant activity of each extract phase of mycelium was higher than that of fruiting body,while the antitumor activity of fruiting body extracts was higher than that of mycelium extracts.There is a significant correlation between antioxidant activity and total flavonoid content.The total flavonoid content of mycelium ethanol extract is higher than that of fruiting body ethanol extract,and therefore fermented mycelia seem feasible to replace fruiting body for production of flavonoids.