摘要
目的制备含镁脱细胞牛骨基质(ABBM-Mg)并对其微观结构和理化性能进行评估。方法将冻干ABBM在2 mol/L镁离子溶液中浸泡7 d获得复合材料,用扫描电镜(SEM)和能谱分析仪(EDS)观察其微观结构并分析元素组成;X射线衍射(XRD)分析复合材料的物相组成;傅里叶红外光谱(FT-IR)检测材料的官能团;电感耦合等离子光谱仪(ICP)检测Mg2+掺入含量以及释放速率,测量压缩强度评价复合材料的力学性能。结果SEM和EDS分析结果显示,在三维多孔的ABBM表面重新沉积了含有镁元素的矿化物;XRD、FT-IR和ICP检测结果表明,通过离子置换法可成功将镁离子与ABBM相结合,且掺入含量为11.67 mg/g;复合镁离子前后材料的压缩强度未产生明显差异(P>0.05)。复合材料中的镁离子体外释放量在4周内可达8%。结论通过离子置换可有效地将Mg2+掺入ABBM中,可实现镁离子缓释效果,并对ABBM的多孔结构和力学性能无明显影响。
Objective To prepare a magnesium-containing acellular bovine bone matrix(acellular bovine bone matrix-Mg,ABBM-Mg)and evaluate its microstructure,physical and chemical properties.Methods The composite material was obtained by immersing the freeze-dried ABBM in a magnesium ion solution at a concentration of 2 mol/L for 7 days.The microstructure was observed under a scanning electron microscope(SEM)and energy spectrum analyzer(EDS)before the element composition was analyzed.The phase composition of the composite material was analyzed by X-ray diffraction(XRD),the functional group of the material was detected by Fourier infrared spectroscopy,the incorporation and release rate of magnesium ions were detected by an inductively coupled plasma spectrometer(ICP),and the compressive strength was measured to evaluate the mechanical properties of the composite material.Results The results of scanning electron microscopy and energy spectrum analysis showed that mineralization containing magnesium was re-deposited on the surface of the three-dimensional porous ABBM.XRD,FT-IR and ICP detection results showed that magnesium ions could be combined with decellularized bovine bones by ion replacement.The matrix was combined,and the content of incorporation was 11.67 mg/g.There was no significant difference in the compressive strength of the material before and after the composite magnesium ion was incorporated(P>0.05).In addition,the in vitro release of magnesium ions in the composite material could reach 8%within 4 weeks.Conclusion This method of ion replacement can effectively incorporate Mg2+into ABBM,which can achieve a sustained release effect of magnesium ions,but has no significant effect on the porous structure or mechanical properties of ABBM.
作者
李彪
李齐宏
胡先同
韩丽伟
赵彦涛
葛成
LI Biao;LI Qi-hong;HU Xian-tong;HAN Li-wei;ZHAO Yan-tao;GE Cheng(PLA 307 Clinical College,Anhui Medical University,Beijing 100071,China;Department of Stomatology,the Fifth Medical Center,Chinese PLA General Hospital,Beijing 100071,China;Beijing Engineering Research Center of Orthopedics Implants,Beijing 100071,China;Department of Orthopedics,the Fourth Medical Center,Chinese PLA General Hospital,Beijing 100048,China)
出处
《军事医学》
CAS
2021年第1期40-44,49,共6页
Military Medical Sciences
基金
军事口腔医学国家重点实验室开放项目(2019KB01)
解放军总医院第四医学中心转化项目(ZH19025)
关键词
脱细胞骨基质
镁元素
离子置换
组织工程
骨移植物
acellular bone matrix
magnesium
ion replacement
tissue engineering
bone graft