纳米孔测序技术在1例呼吸道感染患者病原检测中的应用

Application of nanopore sequencing technology in pathogen detection in a patient with respiratory infection

目的探索快速识别呼吸道感染患者致病病原的方法,为临床早期诊断与治疗提供支持。方法基于纳米孔掌上测序仪MinION对1例患者肺泡灌洗液进行实时测序,用BGI测序平台对样本开展宏基因组测序,并对样本进行病原菌分离培养实验。结果基于纳米孔测序技术的宏基因组测序在55 min内即检测到铜绿假单胞菌特异序列,后续的二代测序及分离培养实验进一步证实了致病病原为铜绿假单胞菌。结论在该例病原检测中,纳米孔测序技术将检测周期缩短至12 h左右,与其他方法相比具有更短的检测周期,有望成为临床感染病原实时检测的重要辅助手段。

Objective To explore a quick method to identify the pathogen of patients with respiratory infection,and to provide support for early clinical diagnosis and treatment.Methods The alveolar lavage fluid of a patient was subjected to real-time sequencing by nanopore sequencer MinION.The sample was further subjected to mNGS sequencing on the BGI sequencing platform before pathogen isolation and culture were performed.Results The specific sequence of Pseudomonas aeruginosa was identified by metagenomic sequencing within 55 minutes based on nanopore sequencing technology.The pathogen isolation and mNGS results were consistent with the results of nanopore sequencing.Conclusion Based on nanopore sequencing technology,the turnaround time can be shortened to about 12 hours in this case.Compared with other methods,nanopore sequencing has a shorter turnaround time,which is expected to be an important supplementary method for real-time detection of clinical infectious pathogens.