玻片固相抗体吸附免疫荧光(SPAIF)在快速检出食品沙门氏菌中的应用

APPLICATION OF THE SLIDE SOLID-PHASE ANTIBODY ADSORPTION IMMUNOFLUORESCENCE (SPAIF) IN RAPID DETECTION OF SALMONELLA IN FOODS

为了排除食品沙门氏菌免疫荧光快速检验中杂菌和杂质对结果判断的干扰,我们应用红细胞吸附免疫荧光技术(EAIF),取得了较普通免疫荧光技术(CIF)明显为好的结果。

The SPAIF technique has been used to detect Salmonella from 354 food samples(104 chickens,100 force-fed ducks,50 fresh porks,50 frozen porks and 50 frozen whole eggs).The SPAIF gave significantly higher positive results than the ordinary immunofluorescence both in the sensitivity and the percentage of agreement between IF technique and routine culture method.The SPAIF has all the advantages possessed by the erythrocyte adsorption immunofluorescence method(EAlF),namely:the staining procedure is simpler;the results of stain are easy to read under the fluorescent micros ope because of cleaner background of view field;and the post-enrichment culture may be omitted;etc,Moreover the sensitivity of SPAIF was higher while the procedure for the preparation of reagents for making smears was much simpler than that of EAIF.Therefore it is more suitable for use in the regional bacteriological laboratories.