狗外周血淋巴细胞转化动力学研究

KINETIC STUDY ON TRANSFORMATION AND PROLIFERATION OF CANINE BLOOD LYMPHOCYTES

自从发现植物血凝素(PHA)能够刺激离体人外周血淋巴细胞分裂增殖以来[1],这一方法被广泛地应用。Ling[2]、Elves[3]已作了较为详细的论述。

Blood lymphoctes of 8 normal dogs were studied to observe the kinetic courses of their transformation and proliferation.The heparinized whole blood cultures were made using a miniculture method in which 0.2 ml blood samples were added to each bottle containing 2.0ml RPMI 1640 medium(containing 20%calf serum)with 300ug PHA,200 u of penicillin and 200 u of streptomycin.Then the mixtures were cultured at 38℃.Serial samples of cells were harvested from the culture at various times(from 0.25 h to 15 days)after incubation in order to observe the morphological transformation of sinai lymphocytes into blast cells,colony formation,mitotic index and total number of cells.The results showed that the course of growth and mitosis of lymphocytes may be divided into three periods,i.e.transformation(up to 2 days),proliferation(2--5 days),degeneration(after 5 days).The kinetic course of transformation-proliferation of canine and human blooc lymphocytes was compared and discussed briefly.The results of this experiment may be important in studying the effect of ionizing radiation and the stimulation of chemical agents on lymphocytes.