人胆汁中胆汁酸的气液色谱分析法

THE DETERMINATION OF HUMAN BILE ACIDS WITH GLC METHOD

气液色谱(GLC)是重要的近代分析手段之一。由于GLC具有分离效能高,能对复杂的多组分混合物进行定性定量分析,因此日益广泛地应用于医药研究中。国外对胆石症患者胆汁中胆汁酸的研究,除采用经典的化学分析法或放射性同位素示踪法外.

For the simultaneous determination of lithocholic,deoxycholic,chenodeoxycholic and cholic acid in the bile of patients with gallstones,the GLC method was adopted.The procedure consists of methylation and acetylation of the sample and subsequent gas chromatographic analysis with a column(2m×3mm)packed with 3%SE-30 on Chromosorb W HP(80--100 mesh)using flame ionization detector.An internal calibration standard of estriohriacetate was introduced.This method was successfully used for the quantitative determination of different kinds of bile acids in human bile.