蚯蚓背肌可能存在“梭曼受体”的证据

EVIDENCES FOR POSSIBLE EXISTENCE OF A "SOMAN RECEPTOR" IN EARTHWORM MUSCLE

起初,我们研究蚯蚓背肌标本的目的是.为了建立一个简易的筛选γ-氨基丁酸(GABA)受体激动剂和拮抗剂的方法[1]。之后,我们又研究了梭曼(GD)对这种标本的影响.

GABA,glutamic acid or soman binding sites could be inactivated or removed from the earthworm muscle cell membranes by means of incubating at 40℃for 50 min,treating with 10%(NH)SOor 1%Triton X-100 for 60 and 30 min respectively without effecting the function of ACh binding sites.ChE activity of the modified muscle peices were found to be much higher than that of the mormal,it indicated that AChE also remained intact on the membrane during the abovementioned treatments.Therefore,musclular contractions induced by ACh were evidently enhanced so long as the enzyme was inhibited after the addition of soman.Soman binding effects could be well distinguished on the superfused muscle preparations from the effects of ACh,GABA and Glu by using bungarovenom or picrotoxin and isopropyl bicyclophosphate or apomorphine as antagonists against these 3 putative neurotrasmitter receptors respectively.Based on the above results,the possible existence of a"soman receptor"in the earthworm muscle was therefore proposed.