摘要
长期以来检查机体细胞免疫力常用两种体内方法——皮肤试验和迟发超敏反应的被动转移试验。然而,体内试验也有缺点[1,2]。因此有必要研究体外检查细胞免疫的方法。体内被布氏活菌苗致敏的淋巴细胞,当体外与该抗原一起培养时,释放各种细胞免疫产物。我们用巨噬细胞移动抑抑试验对巨噬细胞移动抑制因子(MIF)的产生、动态及其与皮肤试验的联系作了探讨.
Guinea pigs were vaccinated with attenuated M 104 strain of Brucella bacilli.Cells of peritoneal exudates were taken for measurement of MIF by capillary technique on different intervals after vaccination.By stimulation of soluble specific antigen,MIF was detected on 11--16th day,approached to the peak on 37th day and persisted longer than 240 days after immunization.The experimental results showed that the sensitivity of macronhage migration inhibition test was compatible to that of skin reaction and could be used for the expression of the cellular immuno-state of the organisms.
出处
《军事医学科学院院刊》
1982年第1期51-54,共4页
Bulletin of the Academy of Military Medical Sciences
