扩散盒血浆凝块培养中E-CFU-D性质的分析

ANALYSIS OF THE PROPERTIES OF E-CFU-D BY IN VIVO CULTURE IN PLASMA CLOT DIFFUSION CHAMBER

造血干细胞(CFU-S)在向骨髓各系细胞分化过程中,存在着--类定向干细胞或造血祖细胞,它们在含有适当刺激因子的体外琼脂或甲基纤维素或血浆凝块培养条件下.

Experimental studies demonstrated that the produced erythroid colonies(E-CFU-D)after 2 days of culture in plasma clot diffusion chamber were neither derived from ERC nor from immature erythroid cells(pronormoblast,early normoblast and intermediate normoblast),but from a subpopulation of erythroid progenitor cells which is in the differentiation pathway between ERC and pronormoblast.Reduction of endogeneous erythropoietin concentration causes immediate suppression of ERC differentiation,but the influence on E-CFU-D is not instantaneously displayed as observed in ERC.However,the experiments of in vivo and in vitro culture show that the proliferation and differentiation of CFU-E or E-GFU-D is also erythropoietin dependant.