日粮脂肪水平对鲤生长、血清生化指标、糖代谢及抗氧化能力的影响

Effects of dietary fat levels on growth,serum biochemical indices,glycometabolism and antioxidant capacity of common carp

为探讨日粮脂肪水平对鲤生长、血清生化指标、糖代谢酶活性及抗氧化能力的影响,本试验配制了3.68%、7.60%、11.52%、15.44%4种日粮脂肪水平。以(17.91±0.16)g幼鲤为研究对象,开展了58 d的饲养试验。结果发现,随着日粮脂肪水平的增加,幼鲤的粗脂肪含量上升,水分含量下降。随日粮脂肪水平的升高,幼鲤血清中甘油三酯、总胆固醇、高密度脂蛋白、低密度脂蛋白含量上升。15.44%日粮脂肪水平幼鲤的6-磷酸果糖激酶、丙酮酸激酶、乳酸脱氢酶活性及乳酸含量均显著高于3.68%日粮脂肪水平幼鲤(P<0.05)。15.44%日粮脂肪水平幼鲤肝胰脏的过氧化氢酶活性显著高于其他3个日粮脂肪水平(P<0.05)。由此可见,日粮脂肪水平过高会导致鲤生长性能下降,影响鱼体组成及糖酵解,造成氧化应激。

In order to study the effects of dietary fat levels on growth,serum biochemical indices,activities of glucose metabolic enzymes and antioxidant capacity of common carp,four dietary fat levels(3.68%,7.60%,11.52%,15.44%)were formulated.A 58-day feeding experiment was carried out with(17.91±0.16)g juvenile common carp as the research object.The results showed that with the increase of dietary fat level,the crude fat content of juvenile common carp increased,while the water content decreased.The contents of serum triglyceride,total cholesterol,high-density lipoprotein and low-density lipoprotein increased with the increase of dietary fat level.The activities of 6-phosphofructokinase,pyruvate kinase,lactate dehydrogenase and lactic acid content of juvenile common carp at 15.44%dietary fat level were significantly higher than those of juvenile common carp at 3.68%dietary fat level(P<0.05).The catalase activity in liver and pancreas of juvenile common carp at 15.44%dietary fat level was significantly higher than that at other three dietary fat levels(P<0.05).Thus,high dietary fat level will lead to the decline of growth performance,affect the body composition and glycolysis,resulting in oxidative stress in common carp.