^(60)COγ线照射后大鼠肝内硷性磷酸酶和葡萄糖-6-磷酸酶变化的组织化学和细胞化学观察

HISTOCHEMICAL AND CYTOCHEMICAL OBSERVATIONS OF AKPase AND G-6-Pase IN RAT LIVER AFTER ^(60)Co RADIATION

用体重250~300g雄性大鼠,研究^(60)Coγ线20 Gy(2000 rad)一次全身照射后,肝内AKPase与G-6-p ase的变化。在照射1、2、3天内将动物断头杀死,取材、恒冷箱切片,用组织化学和细胞化学方法处理。对照组AKPase反应物主要集中于毛细胆管附近,肝小叶周围较小叶中央酶活性显著。G-6-p aseR应物分布于粗面、光面内质网的囊池内和核周间隙内。照射2~3天后AKP ase活性明显增强。照射1天后G-6-p ase的活性略高,2~3天后活性略低。电镜观察与光镜结果基本一致,对上述结果进行了讨论。

Adult male rats weighing 250-300g were used to study AKPase and G-6-Pase in the liver following^(60)Co radiation on the whole body for a single dose 2000 rad(153-156r/min).Irradiated group and control group were sacrificed at intervals of 1,2,3 days after irradiation.The left liver lobe was removed and then cut in a cryostat.AKPase and G-6-Pase activity were revealed by histochemical and cytochemical methods respectively.In the control group.AKPase activity was found to be mainly localized in the vicinity of bile canaliculi.G-6-Pase activity was confined to the dilated cisternae of RER and SER,and to the nuclear envelope.On the 1-3 days after irradiation a markedly increased was found in AKPase activity,while on the Ist day after irradiation there was slightly increased activity in G-6-Pase.On the 2 nd and 3 rd days were slightly decreased.These results were in agreement with cytochemical observations.The significance of these results and related problems were discussed.