摘要
小鼠睾丸组织经研磨制成细胞悬浮液,按常规法分别培养4、24、48、和72小时后制片,统计出生精细胞中终变期、中期细胞所占的百分数,并与未经培养组(对照组1)及活体内注射秋水仙素后取材制片组(对照组2)相比较。结果表明:经4、24小时培养的分裂指数分别为0.44±0.15、1.16±0.52,比对照组1(0.16±0.05)有明显升高;培养4小时组与对照组2(0.42±0.16)相近,但培养24小时组则高于对照组2(P<0.05)。本实验证实:小鼠睾丸细胞在体外经4—24小时的培养,也可达到增加可供染色体分析细胞数的目的。
Meiosis is a special type of cell division that occurs in diploid germ cells du-ring gametogenesis.To study it is of great significance in cytogenetics both theo-ratically and practically.Meiotic cells available for chromosome analysis in usu-ally prepared specimens are very scanty.The present article intends to introduce a simple method to increase the number of cells for chromosome analysis by way of testicular cell culture in vitro.Smears of cell suspension prepared by grinding mouse testicular tissue were made following routine method after 4,24,48 and 72 hours culture respectively.percentages of spermatogenic cells in diakinesis and metaphase were calculated and compared with those uncultivated(control group 1)and specimens from colchicine injected mouse in vivo(control group 2).The results showed that the division index of cells from 24 hours culture was 1.16+0.52,obviously higher than that of control group 1(0.16±0.05)and a little higher than control group 2(0.42±0.16),P<0.05.This experiment demonstrated that the mouse testicular cell may reach a number available for chromosome analysis after 4-24 hours’culture in vitro.
作者
虞世嘉
Yu Shichia(Department of Histology and Embryology,Jiangxi Medical College)
