大白鼠隔区去甲肾上腺素能纤维的起源——逆行荧光标记和荧光组织化学结合法研究

THE ORIGIN OF NORADRENERGIC FIBERS IN THE SEPTUM OF THE RAT——A STUDY OF COMBINED RETROGRADE FLUORESCENT LABELING AND HISTOFLUORESCENCE

本文报道用逆行荧光标记结合Faglu荧光组化法,对21只大白鼠隔区去甲肾上腺素(NA)能纤维的起源进行了研究。结果表明:向隔区投射的NA神经元位于A_(1)、A_(2)和A_(6)群。A_(1)群向隔区的投射呈双侧性;A_(2)和A_(6)的投射则以同侧隔区为主,少数投射到对侧。逆行标记的NA细胞在A_(6)位于其头侧半的背侧部,在A_(1,2)则散在于其全长。A_(4)和A_(7)群内未见标记。有5例在A_(6)群内观察到个别NA细胞被逆行标记。此外在延髓头侧部中缝及腹外网状结构中可见散在的非NA能标记细胞。

21 albino rats were used.The origin of the noradrenergic(NA)fibers in the septum was studied with a simultaneously combined retrograde fluorescent tracing and histofluorescence method.The results revealed that the NA neurons projecting to the septum were in A_(1),A_(2) and A_(6) groups.The projections of A_(1) were bilateral,But A_(2) and A_(6) projected dominantly to the ipsilateral septum,with a few to the contralateral.Retrograde labeled NA cells were localized in the rostrodorsal part of A_(6) group,and scattered over the whole rostrocaudal length of A_(1,2)groups.No labeling was seen in A_(4) and A_(7) groups.In five of the animals,retrograde labeled NA cells were occasionally observed in A_(5) group.In addition,some scattered labeled non-NA neurons were seen in the raphe and the ventrolateral reticular formation of the rostral medulla.