外源性三磷酸腺苷对人胃腺癌SGC-7901细胞的生物学效应

THE BIOLOGICAL EFFECTS OF EXOGENOUS ATP ON HUMAN STOMACH GLANDULAR CANCER CELLS (SGC-7901)

本文报道外源性三磷酸腺苷(ATP)对人胃腺癌SGC-7901细胞系增殖的抑制效应。在SGC-7901细胞的培养基质内加入ATP后,细胞生长明显受阻抑,4天以后,抑制率可达80%以上;癌细胞质膜上的3′,5′环腺苷酸磷酸二酯酶(cAMP-PDEase)活性明显下降,而细胞内的cAMP和纤维粘连蛋白的免疫荧光强度却明显增强。癌细胞多呈梭形,微绒毛减少,表面较光滑,呈现癌细胞向正常方向逆转的表型特征。本文还就ATP作用的机理进行了讨论。

This experiment was carried out on the SGC-7901 cell line in vitro.On the second day of plating,ATP(0.32mg/ml)was added into the culture medium as the experiment group and in the control group only changed the culture medium.The cAMP-PDEase activity and cellular surface feature were observed by electron microscope and scanning electron microscope.The intracellular fibronectin and cAMP were demonstrated by immunocytochemistry method.We have found that ATP not only could inhibit the proliferation of SGC-7901 cells with inhibition rate over 80%after the 4th day of treatment,and decrease the activity of cAMP-PDEase as well as the regression of microvilli on the cell surface but also increase the intensity of specific fluorescence of intracellular fibronectin and cAMP.These results indicated that ATP was a potent inhibitor of cAMP-PDEase of the cell surface of human glandular cancer cell(SGC-7901).The possible mechanism which indicates that ATP might regulate the intracellular cAMP level and thus mediate proliferation and differentiation of the cells was disoussed.