大鼠实验性胃溃疡期间胃窦粘膜上皮及幽门腺的组织化学和放射自显影研究

HISTOCHEMICAL AND AUTORADIOGRAPHIC STUDIES OF THE ANTRAL MUCOUS EPITHELIAL AND GLANDULAR CELLS IN THE RAT DURING EXPERIMENTAL PEPTIC ULCER

用成年雄性Wistar大鼠16只,分为溃疡组和对照组,后者又分为盐水组和空白组。在实验性胃溃疡术后4、6、10和21天取胃窦粘膜,经体外培养,^(3)H-TdR摻入,在放射自显影前后,分别进行PAS反应与醛复红、Alcian蓝(pH2.5)及HE染色,观察胃窦粘膜上皮和幽门腺细胞标记指数、细胞百分率及组织化学变化。正常大鼠胃窦粘膜上皮和幽门腺细胞标记指数为10.03±0.40、细胞百分率为95.73±0.26。溃疡组术后6、10和21天粘膜上皮和腺细胞标记指数增高,与对照组相比有高度显著性差异。21天时新生上皮和腺细胞标记指数增高,与空白组相比,也有高度显著性差异。溃疡组细胞百分率在6天增加,与空白组相比有显著性差异;其中21天与4天和6天相比,均有高度显著性差异。新生粘膜上皮和腺细胞的PAS反应、醛复红和Alcian蓝染色呈强阳性或阳性。本研究的结果提示,实验性胃溃疡自愈期间,胃窦粘膜和新生粘膜上皮及腺细胞增殖活跃,分泌粘液的功能旺盛。上述变化对溃疡局部的修复具有重要作用。

Adult male rats were randomly divided into three groups:the experimental peptic ulcer group,the saline control group and the normal control group.The animals were sacrificed at different time intervals(4-21 days)after the operation.The antral mucosa were removed and were incorporated with^(3)H-TdR in vitro.Paraffin sections were stained with PAS,aldehyde-fuchsin.Alcian blue(pH 2.5),and H.E.before or after autoradiographic procedure.In normal control group,the labeling index of the antral mucosa epithelial and glandular cells was 10.03±0.40,and the percentage of the antral mucosa epithelial and glandular cells was 95.735±0.26.Labeling indexes were raised on the 6 th,10 th and 21 st days in experimental ulcer group after the operation.A significant statistical difference between them and normal control group was found.The labeling index of the regenerated epithelial and glandular cells on the 21 st day was also raised and showed highly significant difference from that of the normal mucosa.The percentage of the antral mucosa epithelial and glandular cells was raised on the 6 th day in experimental ulcer group and revealed significant difference between it and the normal control group.In the experimental peptic ulcer group,the percentages showed highly significant differences on the 21 st day as compared with 4 th or 6 th day.The regenerated epithelial and glandular cells of antral mucosa of the experimental group secreted mucosubstances with strong PAS,aldehyde-fuchsin and Alcian blue reactions.These results suggest that an increased proliferation and secretory activity of the antral mucosa epithelial and glandular cells may play an important role in the recovery of antral mucosa from injury during the experimental peptic ulcer.