人胎造血器官及周围血中体外集落形成单位的分布

THE DISTRIBUTION OF CFU-C IN HUMAN FETAL BONE MARROW, LIVER, SPLEEN AND PERIPHERAL BLOOD

本文采用Metcalf改良的体外单层琼脂培养技术,对40例胎龄3个月到足月非药物引产正常胎儿的骨髓、肝、脾和周围血的体外集落形成单位(CFU-C)进行了研究。本实验表明,1.骨髓在胎龄3个月时已有CFU-C存在,4个月时CFU-C产率达到很高水平,并一直保持到出生前;2.胎肝在4~6月为CFU-C产率的高峰期,7个月迅速下降,到出生前一直处于较低水平;3.胎脾CFU-C水平较低;4.胎儿周围血中CFU-C水平较高,但低于肝和骨髓。

40 human fetuses were used in this study. The range of fetal age varied from 3 months to full terms. All fetuses used were normal and their parents were healthy. The CFU-C from bone marrow, liver, spleen and peripheral blood were studied in vitro with agar culture technique modified by Metcalf.Experiments proved that the CFU-C of bone marrow may be observed in the fetuses in 3rd month, however, it increased rapidly in 4th month and maintained higher level until the end of fetal life. The progenitor cell population was detected at very high level in liver from 4th month to 6th month fetuses. The number of CFU-C of fetal liver were low in 7—10th month. The circulating progenitor cells in peripheral blood of 4, 5 and 6-month fetuses Were on high level but it was lower than that of liver and bone marrow.