大鼠胚心室肌心房肽样物质定位的研究

A STUDY OF LOCALIZATION OF ATRIOPEPTIN-LIKE SUBSTANCE IN HEART VENTRICLES OF RAT EMBRYOS

本文用免疫组织化学方法研究了大鼠胚(13~19天)心房肽样免疫反应物质在心室肌细胞的定位,并在电镜下观察了大鼠胚心室肌细胞的超微结构。所得结果表明,心房肽类物质不仅存在于心房肌,亦在心室肌细胞内。含心房肽样免疫反应物质的细胞主要位于左心室,尤其是靠内膜侧的小梁状或网格状结构内。左右心室壁内这种细胞很少。心室肌细胞内所含心房肽样免疫反应颗粒的数量较同期心房肌细胞少。在电子显微镜下,见部分心室肌细胞含有与心房特殊颗粒(含心房肽类物质)相似的电子致密颗粒,它们大多靠近高尔基复合体。部分细胞不含这种颗粒,前者可能为心房肽样免疫反应阳性细胞,后者可能为阴性细胞。

In this paper the distribution of the atriopeptin-like immunoreactive substance in the ventricles of rat embryos of 13-19 days old was investigated by immunohistochemistry and electron microscopy.The results showed that atriopeptin-like immunoreactive granules were located around the nucleus in some cardiocytes of the ventricles of rat embryos.Most of these cells were distributed in the pectinated or trabecular structures in the luminal surface of left ventricle and a few of them in the myocardium of left and right ventricles.In the same embryo ventricle muscle cells contained less immunoreactive granules than those in the atria.Under electron microscope the atrial specific granule-like granules were found mainly near the Golgi complex.Some cells were devoid of such granules in cytoplasm.In the ventricles the distribution of the muscle cells containing atrial specific granule-like granules corresponds to the sites of muscle cells containing atriopeptin-like substance from the immunohistochemical study.The results suggest that the so-called"atriopeptin"is also present in some ventricular myocytes in rat embryos.The presence of atriopeptin-like substance may be related to the unique type of embryonic circulation.