用血管铸型扫描电镜观察法研究犬左室心壁微血管的构筑

MICROVASCULATURE OF THE LEFT VENTRICULAR WALL OF THE CANINE HEART STUDIED BY CORROSION CAST METHOD UNDER SEM

用8只犬的心脏,从左右冠状动脉注入树酯(甲基丙烯酸甲酯9份加丙烯酸甲酯1份加过氧化苯甲酰)后,置80℃温水中聚合。从左室心壁不同部位取材后,放入50%盐酸中腐蚀,冲洗后在双目镜下选取样品,喷镀后在S-450扫描电镜下观察。在心内膜下观察到方向不同和网眼大小各异的微血管丛;心肌层的毛细血管网由互相平行的毛细血管组成,血管之间可见“H、K和8”型等不同形式的吻合;在铸型样品中可见细小血管与心腔直接相通,可能是心最小静脉;微动脉和微静脉行于肌束间,与肌束垂直配布。毛细血管后微静脉以萝卜根状或以一定间隔接近直角汇入微静脉。在犬左室心壁未见到动静脉分流结构。

Hearts from eight adult dogs were perfused through both coronary arteries with methylmethacrylate.Small specimens cut out from the left ventricular wall were eroded in 50%HCL for 5-7 days.The replicas were studied under the SEM(S-450).The main results were outlined as follows:The subendocardial capillary plexus composed of varied shapes and orientation of meshes was present.The capillary network of the myocardial layers was consisted of many parallel capillaries and between them a variety of models of anastomoses,such as"H""K"and"8"modes etc,were observed.In our findings,it was demonstrated that the arteriole and venule were distributed between the myocardial bundles and their direction was often perpendicular to the myocardial bundles.The postcapillary venules were poured into venule by the"ginger or turnip root"arrangement.The replicas of the Thebesian veins were proved in our specimens.The A-V shunting was not observed.