小鼠乳腺腺癌细胞系MA782/5S-8102细胞的超微结构

ULTRASTRUCTURAL OBSERVATION OF MOUSE MAMMARY ADENOCARCINOMA CELL LINE(MA782/5S-8102)

对小鼠乳腺腺癌细胞系MA782/5 S-8102细胞的超微结构观察,可见细胞基质电子密度不同的亮细胞和暗细胞,细胞核呈明显畸形,核大,核仁数有所增加,核内异染色质浓聚,核膜肿胀,核质比例高。细胞质内核糖体、线粒体显著增加,粗面内质网明显扩张,高尔基体、溶酶体、脂质粒及微丝清楚可见,并观察到胞内A型病毒颗粒及从细胞表面微绒毛芽生的未成熟的B型样颗粒。细胞膜褶皱,细胞表面具有较丰富的微绒毛,在相邻细胞之间可观察到桥粒。结果表明MA782/5 S-8102细胞系细胞具有明显的恶性细胞特征。本文还对所出现的病毒进行了讨论。

The ultrastructural characteristics of the mouse mammary adenocarcinoma cell line(MA782/5S-8102)have been studied by transmission electron microscope.The results show that the cells were irregular in the shape,with abundant microvilli on cell surface.The nucleus was abnormal in shape with the nuclear membrane sunk or swollen,the nucleus/cytoplasmic ratio(N/C)was high.There were many free ribosomes,abundant mitochondrias,dilated rough endoplasmic reticulum in the cytoplasm.Golgi apparatus,lysosomes and lipid droplets were present.Moreover,many cytoplasmic type-A particles and a few immatural type-B particles at the surface of the cell were observed.