大鼠实验性胃溃疡期间胰岛A、B细胞酶组织化学研究

HISTOCHEMICAL STUDY OF ENZYME OF A-AND B-CELLS OF PANCREATIC ISLETS DURING EXPERIMENTAL PEPTICULCER IN RATS

用成年雄性Wistar大鼠65只,分溃疡实验组、盐水对照组和空白对照组。在无菌条件下,将0.01 ml冰醋酸注入动物胃粘膜下层,造成实验性胃溃疡;盐水组模拟手术,注入0.01ml生理盐水;空白组为未经任何处理的正常大鼠。3组动物在相同条件下饲养,在手术后2~28d,分7批取材,取出胰尾进行酶组织化学观察。盐水组:A细胞在手术后2~4 d,AlP活性减弱,5~Nase活性增强,10 d恢复;B细胞在手术后2~4 d,AcP及5-Nase活性增强,ATPase活性减弱,10 d基本恢复。溃疡组:A细胞在手术后2~4d酶活性变化与盐水组相同。手术后6d,AlP、ATPase、SDH、LDH、G-6-PD及α-GPD活性均显示出不同程度增强,至手术后28 d恢复;B细胞在手术后21 d的变化与盐水组基本一致,但手术后4 d,AcP、5-Nase、SDH及LDH活性均减低,21 d恢复。本文实验结果提示,在大鼠实验性胃溃疡期间,胰岛A、B细胞共同参与了机体自然抗病过程。

Sixty five adult male rats were selected for the experiment.The rats were divided into three groups.Peptic ulcer was induced in the experimental group by means of injection of acetic acid to the submucosa of the stomach.Animals of the control group were injected with normal saline instead.Another control group consisted of normal rats without any treatment.All rats were killed at different time intervals(2-28 d)after the operation.The pancreas tissues were prepared for enzyme histochemical study.The results were observed as follows:Two to four days after the peptic ulcer operation,in the pancreatic A-cells,the activity of alkaline phosphatase(A1P)decreased and 5-nucleotidase(5-Nase)activity increased.A1P,adenosinc triphosphatase(ATPase),succinic and lactic dehydrogenases(SDH,LDH),glucose-6-phosphate and a-glycerophosphate dehydrogenases(G-6-PD,orGPD)acuvities were rasied in varying degrees and returned to normal level after 28 days of the operation.In the pancreatic B-cells,during the early stage of the peptic ulcer,acid phosphatase(AcP)and 5-Nase activities increased and ATPase activity declined.But after four days,AcP,5-Nase,SDH and LDH activities declined,then began to recover and returned to normal level after twenty one days of the operation.In the normal saline operation control group,the changes of A-and B-cells,two to four days after the operation were the same as in the peptic ulcer group and returned to normal level during the period of six to ten days.The above histochemical changes showed that the pancreatic A-and B-cells played a certain role in the recovery of the experimental peptic ulcer.