家兔实验性腹膜炎期间腹水细胞的组织化学观察

HISTOCHEMICAL STUDY OF THE EXUDATE CELLS DURING EXPERIMENTAL PERITONITIS IN RABBITS

本文以腹腔注射松节油致腹膜炎后1、3、8、14 d,分别以瑞氏染色和组织化学方法对急性非特异性化学炎症期间(实验组,雄性家兔50只)渗出的腹水细胞和正常家兔(对照组,雄性家兔10只)腹水细胞进行了动态观察。瑞氏染色下,正常家兔腹水细胞为小淋巴细胞(87.1%),少量巨噬细胞(9.7%)和极少数多形核粒细胞。炎症后1 d,多形核粒细胞数目明显增高,3 d后逐渐下降。炎症后巨噬细胞数日逐渐增高,14 d后达到高峰,至30 d后仍未降至正常水平。淋巴细胞于炎症8 d后开始相对增多,14 d后尚未回至正常。组织化学观察:多形核粒细胞的碱性磷酸酶(AlP)活性,于炎症后第l d呈强阳性,3 d开始减弱。巨噬细胞的酸性磷酸酶(AcP)活性,炎症后3 d增强,5 d最强,8 d后开始减弱。脂类反应可见多形核粒细胞与巨噬细胞均呈阳性,巨噬细胞3 d反应增强,同时在胞质内存有未着色的空泡。T淋巴细胞的酸性醋酸萘酯酶(ANAE)反应,5 d时阳性反应率明显增高(从2%升至19.5%)。观察表明,在松节油所致家兔急性非特异性腹膜炎期间各种腹水细胞比例改变,功能增强,提示在炎症期间有促进免疫功能的作用。

Acute non-specific peritonitis was induced by injecting turpentine into rabbits’peritoneal cavity.The exudate cells were collected 1,3,8,and 14 days after the injection from 50 experimental and 10 normal rabbits.Normal peritoneal exudate consists of 87.1%small lymphocytes,9.7%macrophages and small amount of polymorphonuclear granulocytes(PMG).One day after injection the number of PMG increased and fell to normal level 3 days after injection.The number of macrophages gradually increased and reached the peak value 14 days after injection and did not fall to the normal level 30 days after injection.Lymphocytes began to increase in number 8 days after injection and did not return to normal 14 days after injection.Histochemical studies showed that A1P of PMG exhibited strong positive reaction one day after injection and the reaction was weakened 3 days after injection;AcP of macrophages showed increasing activity 3 days after injection which reached maximum on the 5th day and began to decrease after the 8th day.PMG and macrophages were positive for fat staining.Macrophages showed maximal fat reaction 3 days after injection,but contained many vesicles negative to fat stain.Percentages of positively ANAE reactive T lymphocytes raised from 2%to 19.5%5 days after injection.The above results show that during acute non-specific peritonitis induced by turpentine the changes of exudate cell components and histochemical activity may signify that they play a role in immunological function during inflammation.