家兔脊髓灰质细胞构筑的研究

THE CYTOARCHITECTONIC ORGANIZATION OF THE SPINAL CORD IN THE RABBIT

本研究采用14只成年和幼年家兔的各节脊髓,作脊髓灰质分层研究。所用的脊髓切片为80μm、60μm和15μm的横或矢状切片以及2μm的半薄切片。对细胞体和髓鞘染色分别采用甲苯胺蓝、坚牢焦油紫、LuXol Fast Blue及对苯二胺。此外,还用显示灰质乙酰胆碱酯酶活性的方法协助分层研究。分层研究是根据Rexed对猫脊髓灰质分层的原则。研究结果发现家兔脊髓分层基本类似于猫,但在各层的范围及结构方面仍有一定不同。

Fourteen adult and immature rabbits were used for the study of laminar scheme of the gray matter in the spinal cord. The spinal sections were cut transversally or sagitally into 80 μm, 60 μm, 15μm, and 2μm-thick sections. The sections were stained with toluidine blue or crecyl fast violet for cell body and with Luxol fast blue for myelin sheath. 2μm-thick sections were only stained with pphenylenediamine. In addition, the spinal sections from 2 cases of the animals were treated histochemically for demonstrating AChE activity. According to the Rexed’s principle lamination for the cat, we have found that the cytoarchitectonic organization of the rabbit spinal cord was found to be basically similar to that of the cat except for some differences about the extension and structures of the laminae.