大鼠实验性胃溃疡期间胰岛A、B细胞免疫组织化学研究

IMMUNOHISTOCHEMICAL STUDY OF A-AND B-CELLS OF RATS’PANCREATIC ISLETS DURING EXPERIMENTAL PEPTIC ULCER

本实验用成年雄性大鼠40只,分为溃疡实验组、盐水对照组和空白对照组3组。动物在相同条件下饲养。在手术后6、10、14及21 d,分4批取材,进行免疫组织化学染色。用Sternberger PAP法,分别显示胰高血糖素细胞(A细胞)和胰岛素细胞(B细胞),观察大鼠实验性胃溃疡期间胰岛A、B细胞的变化,并进行细胞计数,分别计算A、B细胞所占百分数。正常大鼠A细胞所占比例为23.31±1.91%(X±SD),B细胞为73.15±4.01%;而溃疡组术后10 d,A细胞比例增高达42.67±5.59%,B细胞则为57.59±4.55%。经统计学处理,A细胞比例的增高与盐水和空白对照组相比,均有高度显著性差异(P<0.01);手术后6、14 d,A细胞比例有所增加,但无显著性差异(P>0.05);21 d则基本恢复。本文结果提示,在大鼠实验性胃溃疡期间,胰岛A、B细胞共同参与了机体自然抗疾患的代谢活动,A细胞更为显著。

Forty rats were selected for the experiment,which were divided into three groups including experimental ulcer group,normal saline and normal control groups.The pancreatic tissue were prepared for immunohistochemical staining at 6th,10th,14th,and 21st day after operation.Sternberger’s PAP method was used to show the glucagon cell(A cell)and insulin cell(B cell)in order to observe their changes during the experimental peptic ulcer.In normal control group,the percentage of A cells is 23.31±1.91(X±SD)and that of B cells is 73.1±4.01.But in experimental ulcer group,the A cell percentage is raised up to 42.6±5.59 and the B cell percentage is lowered down to 57.5±4.55 on the 10th day after operation.Comparing with normal saline group and normal group their differences show statistical outstanding differences.The result may point out that both A and B cells of pancreatic islet take part in the body’s natural resistance against the disease,especially A cells.