离体培养鸡胚脑神经细胞的超微结构研究

ULTRASTRUCTURAL STUDY OF DISSOCIATED CHICKEN EMBRYO BRAIN NERVE CELLS CULTURED IN VITRO

本文应用扫描电镜和透射电镜,观察离体培养鸡胚脑细胞的超微结构,侧重描述了神经细胞及其突起间突触连接的形态特征。培养4天后,分化发育成为双极和多极的神经细胞及其突起,彼此已连成网络,并出现初期的突触连接,还见有突触前袋样结构和桥粒样细胞连接。培养7天后,神经细胞之间的轴-树、轴-体和轴-轴等类型的突触连接数目增多,透明突触囊泡密集,结构更完善。培养10天和14天后,神经细胞之间的突触连接仍较多,但已出现神经细胞变性,纤维性神经胶质细胞明显增多。

The ultrastructure of dissociated brain nerve cells from embryonic chicken cult ivated in vitro was studied both under SEM and TEM.Primitive synaptic junctions among the bipolar and multipolar neuronal cell bodies and its processes forming networks of fibers appeared after 4 days of culture.The presynaptic bags and desmosome-like cell junctions were observed simutaneously.Axo-dendritic,axo-somatic and axo-axonal synapses increased in number after 7 days of incubation onwards.Neuronal degeneration and proliferation of fibrous glial cells were evident in10 and 14 days cultures.