大剂量促黄体素释放激素九肽类似物对受孕大鼠黄体影响的组织学和组织化学观察

HISTOLOGICAL AND HISTOCHEMICAL STUDIES OF EFFECTS OF THE HIGH DOSE OF LRH-A ON THE CORPUS LUTEUM OF PREGNANT RATS

本文应用大剂量LRH-A给受孕9d大鼠做腹部皮下注射,观察大鼠黄体的组织学和组织化学变化。给药后24h,观察到黄体退化。油红O染色标本的黄体细胞质内,出现大脂滴,数量很多;3β-HSD酶和SDH酶活性明显下降;少数黄体细胞AcP酶活性增强。48h后,上述变化更加明显,G-6-PD酶活性也有下降。20α-HSD酶活性在对照组与实验组,均为阴性。黄体的毛细血管内皮细胞AlP酶活性,在给药后24h明显升高。提示大剂量LRH-A促使黄体退化,孕酮的合成和分泌功能降低,是造成大鼠在受孕9d全部中止妊娠的主要原因。

In the present experiment the high dose of LRH-A was injected into pregnant rats on Day 9 of pregnancy.Morphology and histochemistry of corpus luteum were studied.It was found that 24 hours after injection LRH-A could effectively cause the corpus luteum to degenarate.A great many large lipid droplets occured in the luteal cells,and the activity of 3β-HSD and of SDH of these cells decreased.At the same time the activity of AcP increased in a few of luteal cells.48 hours after injection the effects outlined above became more distinct,and the activity of G-6-PD also began to decrease.The activity of 20α-HSD in both control and experimental groups was negative 24 hours after injection,while the activity of the AIP increased distinctly in endothelium of the capillary network of corpus luteum.These changes indicated that the high dose of LRH-A had damaged corpus luteum and reduced its synthetic and secretory function of progesterone which,we believe,plays a main role in terminating pregnancy.